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We narrowed to 284 results for: cas9 plasmid

Showing: 161 - 180 of 284 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin... vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 9;520(7546);186-91. doi: 10.1038... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...mutation in dystrophin exon 23. Long et al. used SpCas9 in conjunction with AAV9, whereas Nelson et al....al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively. Although each study...

  2. CRISPR References and Information

    Type
    Collection
    ...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,...KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning... Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122...

  3. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene...flexibility to CRISPR editing. Using a fusion between Cas9 and reverse transcriptase and a prime editing guide..., and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...

  4. Zhang Lab CRISPR Page

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    Collection
    ...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9... Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered...into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...

  5. CRISPR Guide

    Type
    Collection
    ...Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...

  6. Plan Your Experiment

    Type
    Collection
    ...organism without a specific preferred mutation Cas9 (or Cas9 nickase) Single (or dual) gRNA targeting 5′ ...generating a point mutation or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA ...the multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA...concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression...for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in... RNA delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which...generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed...

  7. Call for Guest Bloggers

    Type
    Blog Post
    ...vectors Career development for scientists A new plasmid tool your lab has deposited If you’d like to write...editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI...promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids that provide Cas9 variants...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into...

  9. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...gRNA-containing plasmids (McDade et al, 2016). Many of the gRNAs contained within these plasmids have been ...sequence, Addgene plasmid ID (if we have one associated with the gRNA), application, cas9 species, PubMed...scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet containing... validated gRNAs that are not generated from a plasmid. If you, for example, prefer to deliver your CRISPR...please consider submitting their sequences (and plasmids!) so that this shared resource can continue to... Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and searchable...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...none, need Cas9 plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid... elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should be added...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 ... SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI...

  11. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin..., need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to...vitro transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs...

  12. Zhang Lab's CRISPR Frequently Asked Questions

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    Collection
    ... may also like... Zhang Lab CRISPR plasmids General CRISPR Plasmids Dr. Le Cong, the first author of one...the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...easiest way is to co-transfect several plasmid together, with each plasmid having a targeting spacer cloned ...around 400ng of a typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate...the HR template to avoid this donor plasmid being degraded by Cas9 in cells. One good way is to mutate...around 400ng of a typical size (~5kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR FAQs Zhang Lab's CRISPR Frequently...

  13. Lentiviral Prep Service

    Type
    Collection
    ...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...currently in our repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation...

  14. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...capability of Cas9 to make other types of modifications at specific loci. Activate or Repress : dCas9 fused to...the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target and cleaves...

  16. Worm Expression Resources

    Type
    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits...Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs ...

  17. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast and then selected with...single cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described...

  18. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...HyPer3 (2) which he will co-express together with a plasmid encoding glycine oxidase. The goal is to determine...excited to see how all of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to...

  19. Antibodies 101: Validation

    Type
    Blog Post
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ... the tables below for CRISPR base editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
Showing: 161 - 180 of 284 results