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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 950 results for: cha

Showing: 161 - 180 of 950 results

  1. Molecular Biology Reference

    Type
    Guide
    ...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...amplified) by bacteria, which is an important characteristic of why plasmids are convenient and easy to ...scientists to study gene function as well as identify changes or mutations that may cause certain diseases. Sequencing...termed Sanger sequencing, sometimes referred to as chain-termination sequencing or dideoxy sequencing. To...

  2. Video Library

    Type
    Protocol
    ...Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple...multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple ...

  3. Centrifugation

    Type
    Protocol
    ...directly interchangeable. If you need to convert between the two, use a conversion calculator or chart like...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations). Generate...or follow manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria...

  5. Promoters

    Type
    Guide
    ...transcription actually begins. Only eukaryotes and archaea, however, contain this TATA box. Most prokaryotes...an inducer molecule binds to the repressor, it changes its conformation, preventing its binding to the...

  6. Plan Your Experiment

    Type
    Guide
    ... Edit Generate a specific user-defined sequence change in a particular gene, such as generating a point...constitutively expressed exons, which reduces the chances that the targeted region is removed from the mRNA...

  7. Protocol - How to Design Primers

    Type
    Protocol
    ...Design a Primer You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...concentrations of insert, thereby increasing your chances of success. Set up a vector only control with water...

  18. Cloning

    Type
    Guide
    ...are naturally produced by certain bacteria and archaea, cleave double stranded DNA (dsDNA) at specific...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel, from the archaebacteria Chlamydomonas...light, this channel activates neuronal activity by opening and allowing positively charged ions to rapidly.... Light-gated ion channels directly open and close when exposed to light and change the membrane potential...receptor or pathway.  Light-gated ion channels Light activated ion channels (rhodopsins) either excite or inhibit... range of wavelengths. Excitatory channels allow positively charged ions such as Na+, K+, and Ca++ to ...Tichy et al., 2019), photoswitchable tethered ligands (PTL), and photoswitchable, orthogonal, remotely-tethered...with each light source used.   Channel and activation kinetics Some channels turn on and off very rapidly...

  20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges of succeeding in science...Do-It-Yourself Science” described in Chapter 3, and of course the rapidly changing world of genome engineering ...call bad science.”  The three chapters in this section describe the challenges of trusting science in a “...science’s biggest challenges over the coming years. It is arguably the most subjective chapter of the book,...to. The problems and challenges described in The State of Science have not changed in “COVID times,” but...scientists to change their priorities. For many scientists, COVID-19 will fundamentally change what they ... that may have warranted their own chapters, and certainly chapters that could warrant their own books...
Showing: 161 - 180 of 950 results