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Showing: 161 - 180 of 437 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...Several companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can... mice, you can clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While this post will...development of resistance include (Figure 1): Over-prescription of antibiotics (for example, giving antibiotics...

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  16. The 12 Days of CRISPR

    Type
    Blog Post
    ...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...

  18. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    ... be a few tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar...
Showing: 161 - 180 of 437 results