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We narrowed to 215 results for: crispr cas9 expression plasmids

Showing: 161 - 180 of 215 results

  1. Brzezinski Lab CRISPR Collection

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    Collection
    ...and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic...Schematic of the CRISPR Collection. Plasmids 159654 and 159655 are single guide expressing double stranded...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...

  2. CRISPR Plasmids - Plants

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    Collection
    ...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...CRISPR expression plasmids that have been designed for use in plants. CRISPR... CRISPR Plants CRISPR Plasmids: ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...

  3. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ...Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our CRISPR Guide Check out ...Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors CRISPR 101: Cytosine Transversion...activity. To drive selective A → T editing, they co-expressed Polη, a translesion synthesis polymerase known...inserted the deaminase into the C-terminal domain of Cas9, which narrowed the editing window and reduced A...site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage the cell...engineering going on in the base editing field! From CRISPR-free approaches to mitochondrial base editing to...out our CRISPR 101 ebook ...

  4. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids are available...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI...Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post
    ...RNAs.   RNAi vs. CRISPR: Complementary techniques It is fair to say that CRISPR has revolutionized ...A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...our CRISPR blog posts Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for...transgene. Typically, the desired target of RNAi is expressed as inverted repeats separated by a loop region...unprecedented ways. With the meteoric rise and diversity of CRISPR technologies, one might incorrectly relegate RNAi...straightforwardness of RNAi is unmatched—unlike CRISPR experiments that require exogenous protein, successful...only requires the presence of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt ...

  6. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...tables below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...

  7. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post
    ...organism.   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...snail can be manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated...clutch of eggs? Propagation is thus quite difficult. CRISPR genome editing in squid is possible nonetheless...reptiles at all. But new success is coming with CRISPR/Cas in lizards. The Menke Lab was able to make ...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by injection into ...

  8. Hot Plasmids - August 2020

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page...Addgene #140702) The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using...-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting... Find the plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables...all-in-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for ...approaches rely on continual expression of both dCas9 and the gRNA. This expression can vary significantly ...

  9. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post
    ...dystrophy. While mini-dystrophin expression levels were lower than expression of wild-type dystrophin in normal...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the...Drawbacks: Transgene expression varies with this approach, ranging from ~10% expression in primary human ...this AAV successfully mediated transduction and expression of the full-length transgene in vitro and in ...genomes complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble...increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs (HDR). Split...overlapping split vector has equal or higher transgene expression when compared to using a single over-sized vector...

  10. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ...Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find bacterial...bacterial CRISPR plasmids Read our CRISPR guide ...transposon system. This system has two parts: a plasmid expressing the Tn7 transposition genes (tnsA, tnsB, ...Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid with...different versions of dCas9. Following integration, Mobile-CRISPRi is stably expressed with no selection ...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...

  11. CRISPR Plasmids - gRNAs

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    Collection
    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... any of these gRNA plasmids and review the publication associated with each plasmid for more information...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...

  12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...of interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, ...using synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...sharing, the CRISPR community has created hundreds of useful variants of Cas9. By providing access to the ...Bradbury et al., 2018). Hybridomas can lose gene expression of the mAb-encoding genes or fail to revive after...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...

  13. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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    Blog Post
    ...lactose released activates expression of GFP from the lac operon. GFP expression can then be used to determine...fantastic tools it’s now easy to monitor protein expression, localization, and protein-protein interactions... Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs...Photosactivatable Fluorescent Proteins Use Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent...Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids from the Tsien Lab Browse the Michael Davidson...

  14. Pooled Library Amplifications

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    Blog Post
    ...recombined plasmid to overtake full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional ...How many unique plasmids does your library contain?), the variance of size of plasmids in your library...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...

  15. AAV Q&A with Tim Miles

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    Blog Post
    ... of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus...for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22 in marmosets.... The continued development of more and smaller CRISPR tools is an exciting area for future AAV application...

  16. CRISPR Plasmids - Purify Genomic Loci

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    Collection
    ...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  17. Tips for arabidopsis transformation

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    Blog Post
    ...plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate expression vector...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering....org Find plant plasmids Visit our plant resource page Deposit arabidopsis plasmids with Addgene ...this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium tumeficans - a...options, but here are a couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway...prefer Golden Gate assembly, there are a series of plasmids from the Dinneny lab that additionally allow for...for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and Dinneny, 2013...

  18. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ... multiple plasmids. Second generation packaging systems use 3 plasmids; an envelope plasmid that usually...encodes VSV-G, a packaging plasmid containing structural genes and a transfer plasmid carrying the transfer...Rev gene from the packaging plasmid and incorporating it into a fourth plasmid. In addition, the promoter...and packaging plasmids in order to obtain the best titers. Requiring an additional plasmid for transfection...high titer virus for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest... a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find...By dividing the viral elements across multiple plasmids, the risk of recombination and consequently, the...

  19. Adenoviral Vector Production and Troubleshooting

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    Blog Post
    ... primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions...death of transduced cells and loss of transgene expression (Interestingly, the strong immunogenicity of ...capacity is limited to 8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response... capacity (up to 36 kb!), long-term transgene expression and negligible toxicity. However, their production...down into 5 key steps: Construction of the rAdV plasmid (~1 week): The AdEasyTM system is the most popular...adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest...how to generate the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide...

  20. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging...has a low quantum yield, and when transiently expressed in cells, generates much less signal than comparable...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...
Showing: 161 - 180 of 215 results