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We narrowed to 276 results for: crispr cas9 plasmids

Showing: 161 - 180 of 276 results

  1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.        A retroviral mouse CRISPR knockout library...dependent promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications...library Article contributed by Alycia Bittner CRISPR pooled libraries are popular tools for genetic screens...method used to introduce the gRNAs into cells. Most CRISPR pooled libraries have been created in lentiviral...by lentivirus, and thus screening with available CRISPR libraries was not possible. Primary murine T cells...

  2. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post
    ... Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources... from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility... Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering...Inference of CRISPR Edits) to solve this problem. This tool was initially created to support the CRISPR analysis...successful CRISPR experiments. Jessica is a sci-fi enthusiast, and when she is not guiding CRISPR researchers...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...

  3. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations...specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy not...Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...fragments to create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ...

  4. Editor's Choice, August 2016

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    Blog Post
    ...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...popular blog post on early user experiences with CRISPR alternative, NgAgo. As you might expect with any...up and running in their labs. Pooran surveyed a CRISPR google forum to get a better idea of how many people...Directed Mutagenesis by PCR & Single Base Editing with CRISPR For my editor’s choice picks this month, I’m picking... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

  5. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources ...s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for...A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use viral preps   Improved... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...

  6. Call for Guest Bloggers

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    Blog Post
    ...in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. ... plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid tool your lab ...

  7. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop... DNA Endonuclease Targeted CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave...Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR Collection ...develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both...Together SHERLOCK and DETECTR demonstrate the power of CRISPR-based diagnostics. SHERLOCK- Specific High Sensitivity...Abudayyeh et al 2016). Cas13 can be guided by a single CRISPR RNA (crRNA) to cleave ssRNA or mRNA. It also exhibits...reporters for each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction...

  8. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    Blog Post
    ...our CRISPR resources and CRISPR for plants Bacterial Expression Systems Learn about Global Plasmid Distribution...Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in developing...plant, Dr. Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to...the anti-phage CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in...Addgene-Seeding Labs Plasmid Grant Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen...scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists work on projects...as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant Cry proteins will then be screened...

  9. Plasmids 101: Screens vs. Selections

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    Blog Post
    ... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ....S. Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi...with small molecules/drugs. With the advent of CRISPR, genome-wide knockout screens are possible with...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest...

  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ... Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  ...Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed. Find the Plasmids @Addgene: ...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...

  11. Hot Plasmids: Spring 2025

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ...RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ...

  12. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...publication. Optimizing Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make... an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the...and other types of genome edits, but, even with CRISPR, it isn’t always easy to insert a section of DNA... plasmid...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    Blog Post
    ...time CRISPR user? Check out our tips for 1st time CRISPR users Learn how to Choose the Best Cas9 Variant...Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here Brush Up on Your CRISPR Background...the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ of Regulatory...at hundreds of loci at once (Liu et al., 2020). CRISPR Affinity Purification in situ of Regulatory Elements...CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original...Background with Addgene’s CRISPR Guide Page ... crispr...

  14. Hot Plasmids February 2024

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing...lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due ...multiplexed guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization...Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter...

  15. Pooled Library Amplifications

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    Blog Post
    ...recombined plasmid to overtake full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional ...How many unique plasmids does your library contain?), the variance of size of plasmids in your library...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...

  16. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...Resources on Addgene.org Browse All CRISPR Plasmids Check out our CRISPR Guide Pages ...editing with CRISPR-Cas13.” Science (2017):pii: eaaq0180. PMID: 29070703. Find plasmids from this paper...targeting with CRISPR-Cas13.” Nature 550(7675) (2017):280-284. PMID: 28976959. Find plasmids from this paper... VI-A CRISPR-Cas Enzymes." Mol Cell. 66(3) (2017):373-83. PubMed PMID: 28475872. Find plasmids from this... out our CRISPR Featured Topic Page Learn about RNA Targeting with Cas13a Learn about CRISPR DNA Base ...enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified...C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing...

  17. CRISPR References and Information

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    Collection
    ...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids... cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...

  18. Viral Vectors 101: Viral Applications

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    Blog Post
    ... depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...

  19. Custom CRISPR Screens & the Green Listed Software

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    Blog Post
    ...Learn More About CRISPR Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...world class CRISPR labs.  Check Out This Video to See How Green Listed Works at a Glance!  CRISPR knockout... activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. ...PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for a 1st CRISPR User Resources on Addgene.org...1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green Listed software can ...new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list of genes...

  20. CRISPR Guide

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    ...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genomic regions using CRISPR Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S....References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening... containing the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity can increase the risk...
Showing: 161 - 180 of 276 results