We narrowed to 437 results for: ena

1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ...properties when paired with PHP.eB’s systemic delivery enables minimally-invasive gene delivery and optogenetic...Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics. Nat Methods 16...

2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...stable gene expression? Constant gene expression can enable more confidence in gene expression readouts from...and Christopher A. Voigt. "Engineered promoters enable constant gene expression at any copy number in ...

3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics." ...al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy...

4. Addgene's Tips for Plasmid Quality Control

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   ...Other information may also be critical to the maintenance and use of certain plasmids, such as special ...for the inserts or the full plasmids. This will enable you to quickly BLAST your sequencing result against...

5. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...integrate permanently into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral.... This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ... identify new therapeutic targets/solutions and enable scientists to discover if known drugs can be used...rapid test for the functional activity of VUSs will enable more accurate diagnosis of NFIX-related syndromes...

7. Rabies and Neuronal Tracing

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   ...that blood flows through the heart and lungs and oxygenates the body. However, in neuroscience, this architecture... (e.g., optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal...

8. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   ...with their recent plasmid deposit. These plasmids enable two main types of experiments: Whole mount in ...specific gene expression. The 49 deposited plasmids enable students to study gene expression and functions...

9. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...transfect the cells, these large regions of homology enable us to effectively utilize the host cell’s inherent...tagging with fluorescent proteins as described here enables live cell imaging to study cellular structures ...

10. Viral Vectors 101: Chemogenetics

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    .../10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac, C., & Carnicella, S. (2019). DREADDs: The Power...Olanzapine: A potent agonist at the hM4D(Gi) DREADD amenable to clinical Additional resources on the Addgene...

11. Plasmids 101: The Wide World of Natural Plasmids

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    ...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid...

12. Plasmids 101: Modular Cloning

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    ...what MoClo is and what types of experiments it enables. What is modular cloning? Modular cloning, or MoClo...gene, and terminator.     Fig 1. MoClo enables individual parts to be assembled into larger functional...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...sites.  Step aside, transcriptionally active dioxygenase  When I started to work on building a CRISPR/... TET proteins are not demethylases; they are dioxygenases that oxidize 5-methylcytosine to create rare...

14. Corralling and Cornering the Goldilocks Antibody

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    ...Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility and accuracy while...from antibodies that we screen. This atlas will enable scientists to have an intuitive guide for checking...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...domains of iRFP713. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes... common PAS domain. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes...

16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ...after Nanoblade treatment as measured by their maintenance of pluripotency marker expression and response...Nanoblades targeting the hydroxyphenylpyruvate dioxygenase (HPD) gene, all nine treated mice had ~10% editing...

17. Adapting Toehold Switches to Detect Zika Virus

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    ...Institute (Harvard University), we developed two new enabling technologies for the field of synthetic biology... proof-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of...

18. Cloning Mammalian Cells with the Agarose Method

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    ...that nearly all of the cell lines we tested were amenable to cloning in this manner, we once had difficulty...have prepared two 48-well plates of clones) which enables investigators to identify specific clones with ...

19. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ... Haeussler. With a few extra lines of code, we enabled it to return guides and primers in batch, optimized...Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement...

20. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ..., which sit on the surface of coronaviruses and enable the virus to enter host cells. To do this, they... lab developed a robust in-vitro protocol that enables rapid selection of synthetic nanobodies, called...