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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 369 results for: epor

Showing: 161 - 180 of 369 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...

  2. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab, high school...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  5. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... nuclease active site wasn’t even close to its reported cleavage site on the complementary DNA strand....model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce off-...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    .... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...

  9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining...

  12. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experimental settings. It is a curated set of technical reports on AAV vectors from Addgene and the CLOVER Center...Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity ...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  16. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...even been linked to some diseases. It has been reported that 5-10% of human genes contain a region where...EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the Addgene blog...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    .... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  18. Viral Production at Addgene

    Type
    Blog Post
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...
Showing: 161 - 180 of 369 results