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  3. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    ...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...research project. In total, the interviews lasted from half to all of the day.  This whole process may seem ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves...after transfecting cells, replace the medium with half the volume typically used (e.g., use 5 mL medium...

  12. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...39322032   No terminator is 100% efficient at halting transcription of the template and initiating the...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Turning on the UV light 3-4 times a week for up to half an hour can help keep your hood contamination free...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...incubator, additional growth medium is added to about halfway up each well. Tips for using the agarose cloning...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...understand how protein localization changes over time.Hallmarks of fluorescent protein timers When FP timers...noticed a few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...a travel day to an online call in response to challenges on their end. Though the quick changes in plans...

  20. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...
Showing: 161 - 180 of 429 results