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We narrowed to 532 results for: invs

Showing: 161 - 180 of 532 results

  1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...which are known to be a part of an organelle or involved in intracellular transport pathways, it is possible...direct molecular interaction without further investigation. One fluorescent microscopy method commonly ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...

  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways...a population of mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily...specific classes of human genes. What are the steps involved in a CRISPR screen? Performing a forward genetic...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ... don't have good ways of converting typically invisible information into actionable signals. Let's say...sensors. Those sensors could transduce otherwise invisible molecules into regulation of gene expression including...bioeconomy, and that it’s been 40 years since the invention of genetic engineering. What's incredible is the...

  4. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...process. The majority of current cloning techniques involve the assembly of a circular plasmid in vitro, before...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...

  6. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template that...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  7. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...want the works: organizing reagent storage and inventory, managing orders, tracking students and research...access your protocols at the bench, keep your inventory at your finger tips, and make shopping lists as...protcols, sign-up for shared equipment, and track inventory and orders. Quartzy caters to life science labs...

  9. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...Scrum is that it provides transparency to everyone involved about the status of each project. This creates...example, we recently reduced the number of steps involved during checkout. Demo details for this would be...writing code for common tasks, it would be like re-inventing the wheel over and over again for every single...

  10. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...editor invented. ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE... CBE; the first type of base transition editor invented.   Abasic site A DNA site with the nucleobase...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... This will give you a feeling of what might be involved in such a role. In addition, it can be advantageous...extent there is the need (or opportunity) to get involved in product testing in the lab, to travel to customers...outreach, and more. Technical support roles also involve interacting with other departments such as research...

  13. The Challenges of Cell Culture

    Type
    Blog Post
    ...cell lines can become cross contaminated and are invariably much older than those available from biorepositories... NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification...Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics Guide Abcam Cell Culture ...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ... genes mutated in human cancers have a homolog involved in yeast cell division (Pray, 2008). Scientific...common protein found in lewy bodies), have been invaluable in understanding the basic molecular mechanisms...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

  16. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... of conflicts is important for the individuals involved, and also for the labs, organizations, or businesses... when you know that a person or team should be involved in making a decision and you see no record of ... means the other party will feel punished or invalidated. Both compromise and arbitration might remedy...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... IDLVs were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt...wish you the best of luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus...of SCID-X1 patients. The Journal of Clinical Investigation, 118(9), 3143–3150. https://doi.org/10.1172/...

  18. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...means to manage their work, combining data, team, inventory and project management functionalities with experiment...protocols. To facilitate this, some ELNs allow you to invite people and assign them roles and permission that... all data is organized within an ELN already. Investing some time in deploying a software solution that...

  20. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that their samples...off-target mutations are essential data points for investigators looking to definitively link genotype and phenotype...genome-wide unbiased methods. However, most investigators use single cell clones for in vitro CRISPR experiments...
Showing: 161 - 180 of 532 results