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Showing: 161 - 180 of 204 results

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...PXL 75349 Mammalian U6 yes, activate S. pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from...from pXL yes, activate S. pyogenes EGFP Luikart pRubiC-T2A-Cas9 75347 Mammalian/Retroviral from pXL yes,...yes, activate S. pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry...

  11. mTOR Pathway

    Type
    Collection
    ...provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein...protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR...

  12. AAVED

    Type
    Collection
    ... School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene...Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be...

  13. Viral Production

    Type
    Collection
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ...of inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters...Scientists began to pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli ...

  16. CRISPR References and Information

    Type
    Collection
    ...user. Read more about it on the Addgene blog . Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT... gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+) that can predict gRNA...

  18. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...better for PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

  19. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic...9 system may be re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient...
Showing: 161 - 180 of 204 results