We narrowed to 194 results for: lenti

1. 5 Tips for Troubleshooting Viral Transductions

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   ...out Our Primer for Beginners! Learn All about Lentiviral Vectors Additional Resources on the Addgene....

2. Plasmids 101: Terminators and PolyA signals

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   ... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar...

4. Viral Vectors 101: Producing Your rAAV

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   ...production process.  Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate...

5. Pooled Library Amplifications

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   ...difficult to work with. For example, a library in a lentiviral backbone might be more prone to recombination...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ...

7. Truncated gRNAs for Regulating Gene Expression

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   ... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

8. Hot Plasmids: Winter 2025

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   ...proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ...

9. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...theoretically possible, but likely more challenging, with lentiviral expression. For more detail on how to design ...

10. Lab to Office Culture Shock

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    Blog Post

    ...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize...

11. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular...

12. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post

    ... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ....7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene ...

14. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...

15. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line...

16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...Kidney by Adenovirus, Adeno-Associated Virus, and Lentiviral Vectors After Intravenous and Direct Kidney Injections...

17. CRISPR 101: Off-Target Effects

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    ...been used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the...

18. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive...

19. CRISPR Library Amplification

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    Protocol

    ...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...these sequences are not efficiently packaged into lentiviral particles. See below for options if the recombinant...

20. Plasmids 101: Using Transposons in the Lab

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    Blog Post

    ... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...