Plasmids 101: Terminators and PolyA signals
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Blog Post
... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...
We narrowed to 192 results for: lenti
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Hot Plasmids: Winter 2025
TypeBlog Post...proportions from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...production process. Helper-free production Unlike lentivirus, AAV does not possess the ability to replicate... -
Pooled Library Amplifications
TypeBlog Post...difficult to work with. For example, a library in a lentiviral backbone might be more prone to recombination... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post....org Find Ready-to-Use AAV Find Ready-to-Use Lentivirus Browse Viral Plasmids ... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...theoretically possible, but likely more challenging, with lentiviral expression. For more detail on how to design ... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular... -
Lab to Office Culture Shock
TypeBlog Post...focusing on my technical skills with cloning and lentivirus production, I didn’t really start to recognize... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post... as RNAP II promoters is commonly employed in lentiviral and retroviral constructs and we will discuss... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post....7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...SspB-mCherry-p60 katanin module, which are also available as lentiviral plasmids if you want to make a stable cell line... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post... guide (synthetic, in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive... -
CRISPR 101: Off-Target Effects
TypeBlog Post...been used - GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ... -
CRISPR Library Amplification
TypeProtocol...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...these sequences are not efficiently packaged into lentiviral particles. See below for options if the recombinant... -
Plasmid Collections
TypeCollection...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...