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We narrowed to 174 results for: lentiviral plasmid

Showing: 161 - 174 of 174 results

  1. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...genetically encoded, and luckily, we have pre-designed plasmids for these systems! Ion sensors One common biosensor...we’ve got you covered with packaging plasmids! References and Resources References  Bhalla N., Jolly ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  5. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...ready-to-use AAV preparations of select plasmids; these are noted with the viral service icon . Metal Ions...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin ...

  9. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  10. Viral Vectors

    Type
    Collection
    ... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...are usually divided among many plasmids, such that all of these plasmids must be present for a viral particle...

  11. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 159,124 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins... Find deposited plasmids for specific disease research areas. Cancer Research Plasmids and Resource Michael... Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...

  14. Science Guides

    Type
    Guide
    ...are single preparations of many different plasmids. Plasmids within a given library have the same backbone...about various scientific concepts related to our plasmid collection. Find links to the guides below. Antibodies...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...
Showing: 161 - 174 of 174 results