We narrowed to 275 results for: mel

1. Transferable Skills Guide: Public Speaking

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   ...nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that you ...

2. Giving gRNAs a Facelift - Synthetic and Beyond

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   ....2021.02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

3. RNA Extraction Without A Kit

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   ...within tissues before cell lysis. These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen...

4. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...

5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...antibodies, nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are ...

6. The Future of Research Symposium Boston 2015

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   ...graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director of ...

7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ... ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA...

9. Making CRISPR Plasmids Using Fragmid

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   ...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

10. Antibodies 101: Stripping and Reprobing Western Blots

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    ...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

11. Plasmids 101: Modular Cloning

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    ...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual...

12. Viral Vectors 101: Chemogenetics

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    ...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

13. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...M.K. Montgomery, S.A. Kostas, S.E. Driver, C.C. Mello. Potent and specific genetic interference by double-stranded...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...epitopes are available; this type of ELISA is extremely specific and sensitive to the target protein due...

15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... Melina Fan...

16. Hot Plasmids and Viral Preps - March 2021

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    ...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ...

17. Hot Plasmids and Viral Preps - January 2021

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    ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

18. Fluorescent Proteins 101: Introduction to FRET

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    ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

19. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...

20. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine base editors...