We narrowed to 277 results for: mel

1. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   ...pretty much all investigators. Our kit makes it extremely simple to design TALENs. It only takes two to ...

2. Molecular Biology Reference

   Type

   Guide

   ...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...only sequence short pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid...

3. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   ...nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that you ...

4. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ....2021.02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

5. RNA Extraction Without A Kit

   Type

   Blog Post

   ...within tissues before cell lysis. These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen...

6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   Blog Post

   ...networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...

7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   ...antibodies, nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are ...

8. The Future of Research Symposium Boston 2015

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   Blog Post

   ...graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director of ...

9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   ...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    ... ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA...

11. Making CRISPR Plasmids Using Fragmid

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    Blog Post

    ...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

12. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

13. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual...

14. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    ...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...epitopes are available; this type of ELISA is extremely specific and sensitive to the target protein due...

16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ... Melina Fan...

17. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    ...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ...

18. Tips for Technical Support Calls

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    Blog Post

    ...response, however, consider following up. Ask for a timeline if you are not given one. Though an initial response...

19. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

20. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    ...M.K. Montgomery, S.A. Kostas, S.E. Driver, C.C. Mello. Potent and specific genetic interference by double-stranded...