We narrowed to 279 results for: mel

1. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   ...level. Right away you have something in common. Namely, the scientific question you are interested in ...

2. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...

3. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...pretty much all investigators. Our kit makes it extremely simple to design TALENs. It only takes two to ...

4. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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   ...networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...

5. Transferable Skills Guide: Public Speaking

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   Blog Post

   ...nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that you ...

6. Giving gRNAs a Facelift - Synthetic and Beyond

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   ....2021.02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

7. RNA Extraction Without A Kit

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   Blog Post

   ...within tissues before cell lysis. These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen...

8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...antibodies, nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are ...

9. The Future of Research Symposium Boston 2015

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   ...graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director of ...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

11. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ... ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA...

12. Making CRISPR Plasmids Using Fragmid

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    ...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    ...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

14. Fluorescent Proteins 101: Introduction to FRET

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    ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...

16. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...M.K. Montgomery, S.A. Kostas, S.E. Driver, C.C. Mello. Potent and specific genetic interference by double-stranded...

17. Viral Vectors 101: Chemogenetics

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    ...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

18. Hot Plasmids and Viral Preps - January 2021

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    ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

19. Hot Plasmids - October 2020

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    ...targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...epitopes are available; this type of ELISA is extremely specific and sensitive to the target protein due...