We narrowed to 621 results for: multi

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...recommended that lentiviral supernatants be subjected to multiple freeze-thaw cycles. This protocol outlines the...provide more accurate titers as the likelihood of multiple integration events decreases as the dilution increases...For a more accurate titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were...

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...recommended that lentiviral supernatants be subjected to multiple freeze-thaw cycles. This protocol outlines the...provide more accurate titers as the likelihood of multiple integration events decreases as the dilution increases...For a more accurate titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were...

...Optogenetics Ofer Yizhar New viral prep with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-GFAP-iGABASnFR2(no bind)...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-iGABASnFR2-WPRE AAV1...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-iGABASnFR2(no bind)-...Biosensors GENIE Project New viral prep with multiple serotypes pGP-AAV-syn-flex-iGABASnFR2-WPRE...Biosensors GENIE Project New viral prep with multiple serotypes Looking for more viral vectors... Multiple Authors...

...complex experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary...The inclusion of biological replicates such as multiple mice in each group and C, technical replicates... example, it would be better to treat and test multiple mice with the drug rather than rely on the results...label control, and cross-reactivity control when multiplexing. A, wild-type and knockout samples are the gold... autofluorescence in the sample. D, when using multiple primary antibodies, include controls where each...address the spectral overlap that occurs during multiplexing. Compensation controls are single stained samples... each fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this ...

...DNA molecule (single gene perhaps) to be copied multiple times by Taq Polymerase. From a single copy of...Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix,” which...concentration of PCR product. Master Mix Preparation Multiply the volume of each reagent by the number of individual...different reactions (7 unique primer pairs), so we multiply each volume by 7. In a single 1.5mL tube combine...

...DNA molecule (single gene perhaps) to be copied multiple times by Taq Polymerase. From a single copy of...Sterile dH 2 O (variable) Note: If you are doing multiple PCRs, save time by creating a “master mix,” which...concentration of PCR product. Master Mix Preparation Multiply the volume of each reagent by the number of individual...different reactions (7 unique primer pairs), so we multiply each volume by 7. In a single 1.5mL tube combine...

...antibody) approach?  Staining for multiple targets When staining multiple targets indirectly, you must strategically...Some secondary antibodies are broad and recognize multiple isotypes from a species, like a general anti-mouse... be equally complex with users trying to probe multiple targets in parallel. In these cases, it is critical...cross-reactivity when planning your experiment.  When probing multiple targets in parallel, use the most narrowly specific...staining in parallel    In short, when staining multiple targets, look for primary antibodies raised in...subtype-specific secondary antibodies when staining multiple targets with monoclonal primary antibodies, and...

... pros of multiple channels/balance out cons. Flexible. Can allow people to learn in multiple ways from... (or both!) will be a major limiting factor at multiple points. Before you embark on a project, do your...Podcasts Medium Easy for audience to engage while multitasking. Easy to host guests remotely. Easy access from... possible through other means. Easy to combine multiple types of media. High barrier to entry (people ...from the same experience.  Can combines cons of multiple medias. Switching from one media type to another...another is cognitively demanding. Requires multiple areas of expertise.  Social Media Low to high Variety...

... and simple to multiplex. Problems with FRET kinase reporters Difficult to Multiplex: FRET reporters...easy to multiplex. Their design allows flexible swapping of fluorescent proteins and multiplexing is simple...reporters are challenging to design and difficult to multiplex. The Covert Lab provides an alternative tool with...properties that limit which fluorophores can be multiplexed together. Challenging  to Design: FRET reporter...

...RNA degradation and avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your...

...RNA degradation and avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your...

...modifications in Drosophila using multiple recombinases. To make multiple, precise genome edits, the recombinases...to DNA damage and developmental aberrations. In multiple systems, Cre itself, without the presence of a... high recombinase levels. These data show that multiple recombinases can be used together to designate...for specific recombination patterns that require multiple recombinases. These tools should further enhance...editing capabilities in Drosophila. References: Multiple new site-specific recombinases for use in manipulating...

... achieved by expression of other recombinases. Multiple orthogonal attB-attP pairs per recombinase may...their recognition sites, or by excising one or multiple of their recognition sites from the register. ...primer pair. Then, by leveraging qPCR data from multiple primer pairs, one can infer the predominant state...The qPCR method can be performed on a sample of multiple registers. However, it is only semi-quantitative...is highly degenerate and often times there are multiple register designs that may be used to implement... gene regulation program. We recommend testing multiple register designs for the same gene regulation ...may work faster or more efficiently than others. Multiple large serine recombinases have been described ...

...conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t costain with your...antibodies with the same isotype can not be used for multiplexed indirect staining methods since the secondary...the new Fc region. In addition to increasing multiplexing capabilities this process is frequently used...single-plexed experiments, it needs to be addressed when multiplexing. It is imperative that you use narrowly selective...selective isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above ...and mouse 1gG2a primary antibodies used in a multiplexed imaging experiment   We hope that this blog...

...scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched...easily be engineered. Like humans, mice express multiple subclasses of IgG antibody. Broad range secondary...subclass specific secondary antibodies to detect multiple targets with different mAbs in the same experiment...secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized in a single experiment...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...

...recent advances have allowed scientists to track multiple genomic loci over time using all the colors of...at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building on the work of...al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful ... Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However, they ...publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

...biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST...cases an FP(1–10) fragment may be compatible with multiple FP(11) fragments, even from a different base FP...EBFP2(1–10) and Capri(1–10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...Cerulean Cerulean(1–10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...mRuby4 mRuby4(1–10), pairs with sfCherry2(11) Multiplexed labeling of cellular proteins with split fluorescent...

...downregulate multiple genes at once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac...Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells CRISPR-... deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent stem cells. Previously... construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific Reports Find the...

...single-color labeling; multicolor labeling would enable the visualization of multiple loci. Such a system... that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems...synthetic scaffold that can be used to recruit multiple protein molecules (and also available from Addgene...excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci in human ...

...Screening Using CRISPR/Cas9 Deliver Multiple gRNAs at Once - Multiplex Expression of gRNAs Generating Mouse... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different regions of your gene...reasons to target different regions of the gene with multiple gRNAs, but the most important is that you could...target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different regions of the gene...