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We narrowed to 567 results for: multi

Showing: 161 - 180 of 567 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t costain with your...antibodies with the same isotype can not be used for multiplexed indirect staining methods since the secondary...the new Fc region. In addition to increasing multiplexing capabilities this process is frequently used...single-plexed experiments, it needs to be addressed when multiplexing. It is imperative that you use narrowly selective...selective isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above ...and mouse 1gG2a primary antibodies used in a multiplexed imaging experiment   We hope that this blog...

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...scientists working to visualize multiprotein complexes.  Fig. 2. Multiplex immunolabeling with subclass-switched...easily be engineered. Like humans, mice express multiple subclasses of IgG antibody. Broad range secondary...subclass specific secondary antibodies to detect multiple targets with different mAbs in the same experiment...secondary antibodies and subclassed switched R-mAbs, multiple targets could now be visualized in a single experiment...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...recent advances have allowed scientists to track multiple genomic loci over time using all the colors of...at a range of distances from 2-75 Mbp. Labeling multiple, intrachromosomal loci Building on the work of...al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct colors. To create a colorful ... Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However, they ...publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  4. Kit Free RNA Extraction

    Type
    Protocol
    ...RNA degradation and avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your...

  5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha Multiple FAST Fluorescence-activating...cases an FP(1-10) fragment may be compatible with multiple FP(11) fragments, even from a different base FP...EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split fluorescent...mRuby4 mRuby4(1-10), pairs with sfCherry2(11) Multiplexed labeling of cellular proteins with split fluorescent...

  6. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...downregulate multiple genes at once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac...Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells CRISPR-... deliver uniform and sustained expression of multiplexed gRNAs in human pluripotent stem cells. Previously... construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific Reports Find the...

  7. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...single-color labeling; multicolor labeling would enable the visualization of multiple loci. Such a system... that uses fluorescently labeled Cas9 to label multiple genomic loci in live cells. While other systems...synthetic scaffold that can be used to recruit multiple protein molecules (and also available from Addgene...excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci in human ...

  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Screening Using CRISPR/Cas9 Deliver Multiple gRNAs at Once - Multiplex Expression of gRNAs Generating Mouse... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to target different regions of your gene...reasons to target different regions of the gene with multiple gRNAs, but the most important is that you could...target artifacts by comparing the phenotypes of multiple gRNAs. Targeting different regions of the gene...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...Nagai laboratory has assembled a collection of multicolored nano-lanterns for use in various applications...photobleaching/phototoxicity, they successfully imaged multiple subcellular compartments over several minutes ...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear...Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging.” Proc Natl Acad Sci U S... of bright luminescent protein for real-time multicolour bioimaging.” Nat Commun. 7 (2016): 13718. PMID...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...remedy this, throughout the year we rolled out multiple changes to our website, including the recent homepage... new website search. Tackling findability from multiple angles    We assembled a cross-team team, with...interface.   The team approached the issue from multiple angles: the overall user experience of our website... new search tool, are the results of this multidisciplinary effort and cross-team collaboration (thanks..., human synapsin, hSynap. Matching We capture multiple variations of the same information in our search...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...individual (and multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems...products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid...characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong et al., 2016), in addition...PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM...

  12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...Plasmid Kit Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral ...combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound...system facilitates the simultaneous introduction of multiple genetic alterations into mammalian cells. The ...imaging studies. The ability to simultaneously alter multiple genes via infection with a single ecotropic or...

  13. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...increases. Multiple inserts: There are some cloning methods that make working with multiple inserts a ...as some methods are more amenable to combining multiple DNA fragments than others. In today’s post, we...per reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced cloner...one right answer, so don’t be afraid to explore multiple approaches to find out which one works best for...

  14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ... in detail below [2]. Multipotency: When a cell can differentiate into multiple cell types of the same...[1]. Stem cells’ ability to differentiate into multiple cell types makes them useful models for developmental...hematopoietic) stem cells are a good example of multipotent cells. Unipotency: As the name suggests, cells...ASCs) Also known as somatic stem cells, these multipotent stem cells are located in small quantities in...

  15. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...REPAIR and RESCUE can function in a multiplexed manner to edit multiple RNAs. It’s clear that CRISPR RNA ...research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing systems...This change specifies the edit to be made when multiple As are present in the gRNA spacer, as ADAR will...As discussed above, the ability to edit RNA has multiple advantages, including reversibility and use in...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    ...resistance gene, allowing resistant bacteria to multiply, spreading their resistance genes throughout the...selects for resistant bacteria, allowing them to multiply and propagate their resistance genes. Our society...is hoping to combat antibiotic resistance using multiple approaches. Major restaurant chains including ...antimicrobials, and they suggest that targeting multiple resistance/virulence factors may further reduce...methods. Phage cocktails could be used to target multiple types of pathogenic or drug-resistant bacteria...

  17. Ligation Independent Cloning

    Type
    Protocol
    ...advisable to set up multiple reactions by holding the vector concentration fixed across multiple concentrations...from their recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove...

  18. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...labs prefer to collect and pool the harvests from multiple time points while others opt for a single harvest...transfer and packaging plasmids. A benefit of the multiple harvest strategy is that each day you are supplying...destabilize the viral envelope. One drawback of the multiple harvest approach is that it tends to result in...that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, it is best to...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...mutations. Multiple gRNAs could be used to combat this problem, just as ART includes multiple drugs to ...transcriptional activation. Zhang et al. designed multiple gRNAs to target the 5’ long terminal repeat (LTR...activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well as a latent...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more ...

  20. Gibson Assembly Protocol

    Type
    Protocol
    ...developed a novel method for the easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless...Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single.... Number of fragments assembled simultaneously Multiple fragments can be assembled in one reaction. However...
Showing: 161 - 180 of 567 results