We narrowed to 180 results for: nags
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TypeBlog Post...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded...
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CRISPR 101: Validating Your Genome Edit
TypeBlog Post... may occur, allowing you to Sanger sequence a manageable number of the higher probability locations. If... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...domains that bind flavin (Buckley et al., 2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...opportunities through our career, networking, and management for scientists blog series. We hope to expand... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post....2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & ... -
Finding Your Perfect Job After University
TypeBlog Post... problems can, of course, be solved with good management practices, but can sometimes go unnoticed at ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH... -
Finding Your Science Policy Path
TypeBlog Post...regulatory efforts, policy implementation, program management, communications, diplomacy and international ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...that not every transduced cell will necessarily manage to express CD45. Thus, if transduction efficiency... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...10.1002/anie.201606123 Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, ... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed... -
CRISPR Guide
TypeGuide...SpCas9 3' NGG SpCas9 D1135E variant 3' NGG (reduced NAG binding) SpCas9 VRER variant 3' NGCG SpCas9 EQR variant...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s... -
Addgene's eBook Collection
TypeGuide...career options in science, advice on mentoring and management, resources for identifying your transferable ... -
Chemogenetics Guide
TypeGuide...eaav5282. PMID: 30872534 (Link opens in a new window) Nagai Y, Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, ... -
Optogenetics Guide
TypeGuide... Resource Center Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale, genetically... -
Adenovirus Guide
TypeGuide...) PMID: 17546019 (Link opens in a new window) Matsunaga, W., & Gotoh, A. (2023). Adenovirus as a vector... -
Lentiviral Vector Guide
TypeGuide....72.12.9873-9880.1998 PMID: 9811723 Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997... -
Molecular Biology Reference
TypeGuide...technology, which enables the preparation of more managable (i.e., smaller) DNA fragments. In 1952, Joshua...