... and copy number of the plasmid) and typically include a gene that is advantageous for survival, such ...range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene knock-down...the many things that plasmids can be used to do include: Produce large amounts of a protein so that scientists...the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose...

... Now you’re wondering if your favorite gene is included in other CRISPR screens and whether that data ... each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system, sgRNA...-depth walk through of how to use ORCS:     Include your CRISPR screen data in ORCS  Researchers who...who wish to have their CRISPR screen data included in the BioGRID ORCS CRISPR database are encouraged ...

... lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic domains, as well as...as the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com...the cleavage domains, the recognition domains—including the CTD and the entire REC lobe—do not structurally...lobes. The guide RNA in this structure does not include stem loop 3, and guide RNA nucleotides 11-20 are...discoveries on this topic are still being made! Conclusion Finally, we can summarize the process from start...

...building blocks including 14 different fluorescent proteins, 8 constitutive promoters, 2 includible promoters...Characteristics of the pHlash reporter protein include: H+ specific response cytoplasmic retention insensitivity...been used for a variety of experimental purposes including (1) single or multiple, constitutive or inducible...MXS-chaining were used in a variety of experiments including subcellular visualization of 6 different organelles...introduction of many different functional groups, including reactive groups for conjugation reactions, fluorescent... characteristics favorable for innocuous tags including high solubility, limited affinity for other proteins...mammalian cells with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your...

...distributing lentivirus (with many CRISPR tools included among the preps that are currently available) ... AAV preparations have worked well in my lab, including in applications where we have delivered them in...The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot...on each viral prep’s specific material page and includes western blots as well as other expression data...

... browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several... for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. ...

...span four continents and research diverse topics including microbiology, brain injury, drug discovery, and...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering...studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, and recently...fluorescent signals from the sensor, and send a signal including its position in the river back to the user.   ...

...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

...GCE, each component of the experiment system (including media, cell type, and growth temperature) may ...to use a protocol that you’ll have to rework to include reagents accessible to your lab. The solution GCE...is for recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses... While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol, to optimize...

...ways foreign DNA can be introduced into cells including transformation, transduction, conjugation, and...often comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the...Addgene. For more information on viral vectors, including transduction download our Viral Vectors 101 eBook...into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab....

...example, Addgene’s contributions by Tyler Ford included reagent sharing and were presented at the ASPB...at this specific conference, and I was able to include and exclude different modules for this workshop...learned: Since early 2018 the team has grown to include experts who could help provide new materials and...assembled the right people at the right time. Be inclusive and seek solutions by working with communities...

...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

.... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

...are required for any application. If you want to include a homology directed repair (HDR) template to create... table below summarizes the various components included with each expression system as well as suitable...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...Difficulty Hard Hard Hard These tables are not inclusive of all methods, nor are these methods limited ...

...addressed in a somewhat cursory manner, their inclusion here adds much needed context to understand every...to see the dirty laundry exposed here, but it’s unclear how much they will actually care. The section of...state of science would be complete without the inclusion of some interesting case studies, and Zimmer provides...who originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing...