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We narrowed to 466 results for: organization Addgene

Showing: 161 - 180 of 466 results

  1. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ... and have the biggest impact. We should know — Addgene has built its foundation on this idea! And we believe...partnerships with like-minded individuals and organizations, like the Allen Institute, to achieve greater...together. The Allen Institute is a research organization that is committed to understanding some of the...high specificity and brightness, now available at Addgene!   {% module_block module "widget_58e2d882-2fb6...experimental requirements. Best-in-class collection at Addgene The GTA contains data on thousands of AAV enhancer...enhancer plasmids, which are all available at Addgene. This allows scientists to choose enhancers that may... or target a very specific cell type. However, Addgene has partnered with the AIBS to showcase the “best-in-class...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...particular organism, and the library is only compatible with cells derived from that organism. All of our...pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

  3. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ... up will be easy. Additional resources on the Addgene blog Browse other blog posts on networking Attending...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on the...

  6. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

  8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies...

  9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ... C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed PMID: 30704911...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters, check ...assembly step. Find the miRNA reporter collection at Addgene! Gam JJ et al., Nat Commun, 2019. PubMed PMID: ...plasmids at Addgene. LaFleur et al., Nat Commun. 2019, PubMed PMID: 30971695. ...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...plasmids or plant expression systems available from Addgene. If you have any questions or thoughts about this...PubMed. Find the plasmids from this publication at Addgene Other Techniques for gRNA Multiplexing Multiplexed....  Find the plasmids from this publication at Addgene Dimeric CRISPR RNA-guided FokI nucleases for..... Find the plasmids from this publication at Addgene ...

  11. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...excellent questions.    Additional Resources on the Addgene Blog Using virus in your research - a primer for...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  13. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised... Turnover at Addgene is about half of what is standard in most sectors. The Addgenies support a high... ahead of the curve on this. Addgene’s success is about the Addgenies. I never worry about their dedication...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...

  14. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and...

  17. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...handful of Click Biology tools available at Addgene. Addgene Link Click Biology Tag Description Humberg...already deposited at Addgene in multiple formats: for use in different organisms, targeted to particular... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...by dialing up the right nucleic acid sequence (Addgene can help with this!). Besides requiring stable ...signaling. Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding ...s41589-025-01944-x   Additional Resources on the Addgene Blog Live and Let Dye: Self-Labeling Protein Tags...Plasmids 101: Degron Tags Additional Resources on addgene.org New England Biolabs Cell-Imaging Plasmid Collection...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Services  Resources on the Addgene blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

  20. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...shipped 2,078 items to 764 scientists at 375 organizations in 21 different countries. It's safe to say ...publication by pasting the paper's title into the Addgene website search bar?   We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...
Showing: 161 - 180 of 466 results