...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...doi.org/10.1002/iub.165  Additional resources on the Addgene Blog CRISPR libraries Genome-wide screens with...

...in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern...used to generate mutations in a wide range of organisms, but its potential to efficiently correct disease..., however, because the disease affects multiple organ systems. Nevertheless, the findings are proof-of-principle...functional correction of the gene in clonally expanded organoids. “CRISPR in adult stem cells has huge potential...potential, because the genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...excellent questions.    Additional Resources on the Addgene Blog Using virus in your research - a primer for...Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised... Turnover at Addgene is about half of what is standard in most sectors. The Addgenies support a high... ahead of the curve on this. Addgene’s success is about the Addgenies. I never worry about their dedication...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...

...size have allowed scientists to use it as a model organism to interrogate many scientific questions. But ...demonstrate the new system in a video filmed at Addgene:     If you're working in fly research, I hope...References and Resources Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References...

...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and...

...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Services  Resources on the Addgene blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...shipped 2,078 items to 764 scientists at 375 organizations in 21 different countries. It's safe to say ...publication by pasting the paper's title into the Addgene website search bar?   We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...

...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing... Various Addgenies...

...to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists share...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...

...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...based biosensors.     Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

...Additional Resources on the Addgene Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their.... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...scientific knowledge. On this new page, we have neatly organized the educational video content from our website...or to answer any questions you might have about Addgene. The page is divided into three main sections: ...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...

...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping...  Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order...Suggest A Plasmid You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which...tech transfer team will communicate with your organization’s technology transfer office or other authorized...

...materials. Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...contact our friendly customer support team at help@addgene.org.  Curious about how rabies plasmids are used...

... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, ...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ... talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the...conference was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking...

...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...  Find plasmids for your research Learn about Addgene's viral service ..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...component available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...the ID to look up the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid...

...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...cellular level instead of having to work with a whole organism and can help scientists produce vital tools in...website Watch Addgene's video collection ...