We narrowed to 400 results for: pho;
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TypeBlog Post...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...Institutes of Health endanger scientists’ ability to uphold their side of the bargain and do the research required...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...
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Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily scored. However, determining... a targeting sequence for the adenine phosphoribosyltransferase (APT) gene, which is conserved in many... -
CRISPR History and Development for Genome Engineering
TypeCollection...sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post... Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33...Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem... Deaf Patient With MYO7A Mutation Results in Morphologic and Functional Recovery of the Derived Hair Cell-Like... -
Delivery Methods for Generating iPSCs
TypeBlog Post...the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine...pluripotency, but they also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should...little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins, and promoter... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog Post...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it. All photos tagged with #DeckTheLab throughout the month of... -
Addgene's 20th Anniversary Party
TypeBlog Post...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable... -
Using AAV for Neuronal Tracing
TypeBlog Post...of application and aid in visualization of the morphology of individual neurons, including dendritic and...vivo, microinjection, pressure injection or iontophoresis (applying small electric currents via inserted... project but neurons were traced with AAV2. Two-Photon imaging was used to generate a 3D finemap of neurons... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog Post...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate... -
Editor's Choice, September 2016
TypeBlog Post...and provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When ...the chance to stop what you’re doing and make a phone call or write out an email. You need help right ... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post... Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red, yellow, cyan... (e.g., apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just... -
An Addgene Summer Internship
TypeBlog Post...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens.... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time...vesicles are primarily used by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy...