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We narrowed to 416 results for: plasmids 101

Showing: 161 - 180 of 416 results

  1. Retrovirus Plasmids

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    Collection
    ...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ... — including the recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

  3. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  4. What's New in CRISPR - November 2021

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    Blog Post
    ... the PEDAR plasmids   Additional resources on the Addgene blog Download the CRISPR 101 eBook Find ...guide Find CRISPR plasmids by function Find recently deposited plasmids Topics: CRISPR, Other CRISPR Tools... where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more...will cover: Miniature CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas...Read the paper in Molecular Cell Find the CasMINI plasmids Efficient CRISPR editing with a hypercompact ...paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f...cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in...

  5. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime ...CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids for base...overview with our CRISPR Guide Check out our CRISPR 101 ebook ...

  6. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ... https://doi.org/10.1016/j.xgen.2024.100519 More resources from Addgene Plasmids 101: Modular Cloning ...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because... Mammalian Plasmid pRDA_791 (contains only orio and ampR) Drosophilia Plasmid pRDB_051 (for...the assembled plasmid. It shows you each component, with the ID of the component plasmid, component name...the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating...Cloning Addgene's CRISPR Guide Plasmids 101: Golden Gate Cloning...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101: Systemic...Request Browse all in-stock AAV preps Browse all AAV plasmids .... Cell, S0092-8674(25)00692-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, C. L., Allen, J. M....Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters, R. W., Yi, S. M.,...276(23), 20610–20616. https://doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006... Gene Therapy, 14(3), 316–327. https://doi.org/10.1016/j.ymthe.2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna...

  8. Viral Vectors 101: Systemic Capsids

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    Blog Post
    ...Addgene blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to AAV References...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer), ...AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic capsids are easy to produce...produce in high titer, but higher doses (>1 x 1012 vg total in mice) may increase the chances of inducing... At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater number...

  9. Tips for Using BLAST to Verify Plasmids

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    Blog Post
    ...Addgene Blog: Plasmids 101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality ...results of the plasmids in our repository with known reference sequences, such as full plasmid sequences provided...up their plasmid and cloning verification steps and free up more time for using your plasmids instead!...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid...provided by the laboratories that deposit their plasmids with us or other entries in NCBI’s numerous databases... grown over the years (we now have over 60,000 plasmids!), the number of sequencing results we analyze...busy week, we may need to analyze more than 200 plasmids as part of our quality control process. Consequently...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene? Check... resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables... requested plasmids. I wondered how many scientists have used the protocol for this plasmid, and if it... your protocols onto your plasmid page, scientists who request your plasmid will have easy access to your...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...deposit their plasmids to the repository. These protocols show up on the related plasmid pages and can...http://www.addgene.org/ If you’re working with plasmids and you decide to share them using Addgene’s repository...

  11. Viral Vector Guides and Plasmids

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    Collection
    ...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are ...genes and divided into multi-plasmid systems. These systems consist of a plasmid containing the genetic information...be delivered (transfer or shuttle plasmid) and one or more plasmids containing the necessary components...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...required genes are divided in the multi-plasmid system. Many viral plasmids are also self-inactivating, meaning...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...

  12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post
    ...interest has been cloned into an expression plasmid, the plasmid can be introduced into host cells, such ...that the plasmids and sequences for antibodies are not shared. For instance, through open plasmid sharing...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: What...Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing one ...synthetic genes that are typically expressed from a plasmid or from an integrated sequence in a stable cell...heavy chain genes of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an...laboratories and companies have already begun creating plasmids encoding affinity reagents. These include conventional...

  13. New FLIS Plasmid (April Fools 2022)

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    Blog Post
    ...inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited a plasmid that will forever change...blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based system...

  14. Deisseroth INTRSECT Collection

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    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

  15. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

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    Blog Post
    ... after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you begin your...with 210 plasmids from the collection. You can find the full list here. Most of these plasmids are empty...the Addgene blog Browse blog posts from our Plasmids 101 blog series Learn about the different grades...cloning experiment? What if after receiving the plasmid from Addgene you could directly start your digest...you can focus on what you want to do with your plasmid without spending time and resources in preparative...research and discovery.” Here are the details. Plasmids offered as cloning grade DNA As we begin testing...molecular cloning. Plus, we decided to include 10 plasmids that can be directly used in the research on the...

  16. 5 Science Rap Videos & Their Creator: Tom McFadden

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    Blog Post
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org ...

  18. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ...lentiviral plasmids include popular envelope and packaging plasmids as well as transfer plasmids. Download...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.  Download...background.         If you’re predominantly a plasmid user, instead, don’t fret; we have backgrounds ...

  19. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post
    ... Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...protein fusions Browse the fluorescent proteins plasmid kits ...

  20. Plasmid Preps: Different Purity, Different Quantities, Different Uses

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    Blog Post
    ...Addgene blog Browse blog posts on plasmid cloning Read our Plasmids 101 blog posts Learn about the differences...Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important... into a plasmid backbone. Transfection grade DNA Figure 3: Need to introduce plasmids into mammalian... an external plasmid in mammalian cells and it will likely require micrograms of plasmid DNA for each ...utilize your plasmid DNA, so that you can decide what will be the optimal type of plasmid preparation ...purity. Based on these premises we can classify a plasmid preparation in 3 different ways: Transformation...Transformation introduces transformation grade plasmids into a bacterial cell. Transformation grade...
Showing: 161 - 180 of 416 results