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We narrowed to 361 results for: reporter

Showing: 161 - 180 of 361 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    .... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...even been linked to some diseases. It has been reported that 5-10% of human genes contain a region where...EMBO Rep 1:18–23 . https://doi.org/10.1093/embo-reports/kvd001 Additional resources on the Addgene blog...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...

  4. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...

  5. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  6. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  7. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

  9. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...

  14. New Viral Vectors - March 2024

    Type
    Blog Post
    ...check out Addgene's Data Hub to view viral vector reports from other users. And don't forgot to use the link...

  16. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create this lovely...

  18. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...

  19. Tetracycline Inducible Expression

    Type
    Collection
    ...shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping...

  20. CRISPR Plasmids - Tagging

    Type
    Collection
    ...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...
Showing: 161 - 180 of 361 results