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We narrowed to 363 results for: reporter

Showing: 161 - 180 of 363 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    .... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  2. Viral Production at Addgene

    Type
    Blog Post
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  3. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    .... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either... well of your cells. Regardless, the amount of reporter protein activity correlates to both the amount...

  8. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...

  9. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...

  13. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create this lovely...

  15. New Viral Vectors - March 2024

    Type
    Blog Post
    ...check out Addgene's Data Hub to view viral vector reports from other users. And don't forgot to use the link...

  17. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... veritable virus factory.  Their findings were reported in the Journal of Virology.  The EBOV glycoprotein...
Showing: 161 - 180 of 363 results