We narrowed to 304 results for: ski
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TypeBlog Post...designed with the help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the ...e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program...
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Plasmids 101: Biotinylation
TypeBlog Post...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005. Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution... -
Hot Plasmids: Spring 2025
TypeBlog Post...designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed...Aggarwal, A., Negrean, A., Chen, Y., Iyer, R., … Podgorski, K. (2025). Glutamate indicators with increased... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...doi.org/10.3390/cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...10.3390/pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-... -
Plasmids 101: Restriction Cloning
TypeBlog Post... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...maintain partial function due to the use of exon skipping, alternative start codons, and/or the phenomenon...Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, Michon A-M, Sun H, Tessmer... -
Antibodies 101: Validation
TypeBlog Post...this sample type, so you use your bioinformatics skills (or your friendly neighborhood bioinformatician...//doi.org/10.1016/j.nbt.2018.08.002 Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...would improve my scientific and application writing skills, and ended up with the award. We are often more...These applications can be highly competitive, so asking for help from mentors and other students who have... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...complementary edits to opposite strands of the DNA and skip the cellular mismatch repair step (Anzalone et al..., M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L., Zhou, W., Jiang... -
Antibody Validation for Flow Cytometry
TypeBlog Post..., K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P.,...https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...translational complex assembly. PhD student Dejan Gagoski then used SITS to create the pCell-Free vectors...both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed a library ...systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set... -
Introducing...Addgene Quick Tips!
TypeBlog Post...tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new tip every ... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...recently chosen four names as finalists. Now, we’re asking you to help us make the decision between some meaningful... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...our library more useful to our community, we’re asking you for plasmid suggestions. Can’t find a plasmid... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You... -
Congrats to the 2019 Deck The Lab Winners
TypeBlog Post...Cytology group! @EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM... -
Addgene at SfN23!
TypeBlog Post...if you're looking to brush up on your conference skills, check out some of our helpful conference blog ... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post... cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness...