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Showing: 161 - 180 of 304 results
  1. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...designed with the help of the DNA tuner program) (Latynski and Valentovich 2014). We routinely clone the ...e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer program...
  2. Plasmids 101: Biotinylation

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    Blog Post
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...
  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution...
  4. Hot Plasmids: Spring 2025

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    Blog Post
    ...designed and improved by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed...Aggarwal, A., Negrean, A., Chen, Y., Iyer, R., … Podgorski, K. (2025). Glutamate indicators with increased...
  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    ...doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes....2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R. J. (2006). Α2,3 and α2,6 N-Linked Sialic Acids...
  6. Viral Vectors 101: AAV Variables That Matter

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    Blog Post
    ...doi.org/10.3390/cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...10.3390/pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...
  7. Plasmids 101: Restriction Cloning

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    Blog Post
    ... using UV as it can cause damage to the eyes and skin. When running a gel for purification purposes it...wide gel comb, run the gel on the slower side, and skip lanes between samples. In addition to a DNA ladder...
  8. Antibodies 101: Validation

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    Blog Post
    ...this sample type, so you use your bioinformatics skills (or your friendly neighborhood bioinformatician...//doi.org/10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E...
  9. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ...would improve my scientific and application writing skills, and ended up with the award. We are often more...These applications can be highly competitive, so asking for help from mentors and other students who have...
  10. Antibody Validation for Flow Cytometry

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    Blog Post
    ..., K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P.,...https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg...
  11. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...translational complex assembly. PhD student Dejan Gagoski then used SITS to create the pCell-Free vectors...both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He has also constructed a library ...systems: E. coli, wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set...
  12. Addgene at SfN23!

    Type
    Blog Post
    ...if you're looking to brush up on your conference skills, check out some of our helpful conference blog ...
Showing: 161 - 180 of 304 results