...to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are advantageous because they do...

...clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid. It can take...

...performing a single base pair edit with a prime editor. What next? When generating a knock-in mutation...

...sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can ...Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you will need to...which orientation the insert is ligated into the vector backbone and therefore the only thing left to do...

...cut the vector, you will need to use a phosphatase to prevent re-circularization of the vector if you ...having difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut ...

...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of... up your work area and microscope after use and store the microscope appropriately....

...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility...

...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She...

...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified...