We narrowed to 1,073 results for: TOR;

...uses to differentiate between variations in those factors. To understand the principles of gating, you’ll.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

... RNA pool was cloned into bacterial expression vectors to create a library. Bacteria were transformed,...Jaffrey group was able to quantify the effect of mTOR inhibition on the activity of RNA Polymerase III...

...break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen species, ...plasmids that liberate the targeting region from the vector. Alternatively, PCR-generated or commercially synthesized...

...to listen, and an ability to remember the right stories at the right time. Like many of the posts in our...another job, it will be important to retain the stories and examples that have guided your resolution processes...

... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively... mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend targeting...

... a certain region or calculate a colocalization factor to assess the spatial relationship between the ...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...

...Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9 either through injection...Mediated Genome Editing with Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci...

...further lower fidelity, like changing the metal cofactor and the dNTP ratio. The resulting random errors...Addgene blog All Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing...

...expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells...gift from Feng Zhang and is described in Improved vectors and genome-wide libraries for CRISPR screening....

...PA-GFP. PA-GFP was derived from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine..."Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging." Nature methods...