...Resources Viral Vector Production Learn about how we produce high-quality viral vector preps at Addgene...Request service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification... Viral Vector Packaging Service Packaged on Request AAV Packaged...Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively... protection levels. We offer 963 in-stock viral vector preps, delivering high quality and ready availability...availability at affordable prices. While our repository serves the needs of many researchers, scientists sometimes... the steps to produce, purify, and titer viral vectors. Overview Our new Packaged on Request service is...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus... made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...receive a ready-to-use viral preparation from the inventory below. Lentiviruses are distributed as purified... that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific...

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...tagging system were tested by tagging transcription factors with FLAG in human cell lines. To repeat the tagging...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...

...Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search based on factors such...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...webtool that uses deep learning models for base editor gRNA design and efficiency prediction. Developed...webtool that uses deep learning models for prime editor pegRNA design and efficiency prediction. Developed... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...information from Cas-OFFinder and Microhomology predictor. Developed by the Jin-Soo Kim Lab . CRISPR-ERA...

...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium Community Resources...including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic...Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins...Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks BioBricks Foundation...

... in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene (e.g. GFP...or inserting a tag Cas9 (or Cas9 nickase); Base editor Single (or dual) gRNA targeting the region where...reduced efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...promoter elements of target gene Many different activators exist, including the multi-plasmid SAM system. Select...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...

...to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor molecule...and measuring the emission of the acceptor. For a given donor-acceptor pair, the FRET efficiency strongly...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET...proteins have been developed for use as donor and acceptor pairs in FRET experiments. The efficiency of ... depends on the distance between the donor and acceptor molecules and can therefore be used to calculate...calculate the distances between the donor and acceptor. FRET is often used to study (1) protein-protein interactions...each protein is separately fused to a donor or acceptor molecule (also referred to as intermolecular or...

...Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith... developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL ...2012). All three of these platforms assemble TAL effector repeat arrays on a single architectural framework...all three methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...

...Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification...encoding tet-off transactivators and tools for affinity purification (TRAP). Viral... Viral Vector Packaging Service...Neurophysiology Cell Ablation Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...