.... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

...Two classes of base editors — cytosine base editors (CBEs) and adenine base editors (ABEs) — can be used...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... without double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically...dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and...on the opposite strand). Likewise, adenine base editors have been engineered to convert adenosine to inosine...Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further...

...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °... antibodies should not be stored in sodium azide, as it acts as an inhibitor to HRP. In those cases, thimerosal...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...expensive than polyclonal antibodies. Environmental factors, binding partners, location in the cell, and sample... a primary antibody, which becomes the limiting factor for the number of proteins you can detect in a ...

...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration...Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in ...lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc -...

...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian...Find prime editor plasmids for (search and replace) genome editing. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...enzyme is named PE1 . PE1 and most other prime editors use the Moloney murine leukemia virus (M-MLV) reverse...can be used in PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition...addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA -...

...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

...support educators interested in integrating MDH-related research into their teaching laboratories. They ...access to mentors, virtual and local professional development, membership to a local hub of educators teaching... Depositor Collections MDH CUREs Community Malate Dehydrogenase...They encourage instructors teaching different types of courses, at any type of institution, and at different...teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a ...a new window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to...cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of ...

... Viral Vector Packaging Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics...information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory hM4D(Gi) DREADD KOR DREADD...Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion...AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...