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We narrowed to 971 results for: lars;

Showing: 951 - 960 of 971 results

  3. Immunocytochemistry

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...

  4. Colony Formation Titering Assay

    Type
    Protocol
    ...cells for mycoplasma (Link opens in a new window) regularly Do not over or under-grow your cells. Thaw a new...

  6. CRISPR Guide

    Type
    Guide
    ...both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species of CRISPR enzymes... Browse Plasmids: Prime Edit Large Scale Edits CRISPR can generate large-scale genetic changes, including...Cas13a-based molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular detection...CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion Tools Cas9...techniques are referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies...and adaptability, CRISPR rapidly became the most popular genome engineering approach. Figure 1: Overview...knockout or gene edit; or using Cas9 to generate large genomic deletions or inversions with two gRNA sites...

  7. Cloning

    Type
    Guide
    ... Molecular Biology Reference Choosing a Molecular Cloning Technique Molecular Cloning Techniques...Addgene's reference guide on molecular cloning techniques, including information on seven commonly used... Ligation Independent Cloning Oligo Stitching Molecular cloning or the creation of recombinant DNA is ...used in scientific research and discovery. With molecular cloning scientists can amplify and manipulate ...following guide will highlight several of the most popular cloning methods used to create recombinant DNA....Cloning Restriction enzyme (endonuclease) based molecular cloning is the "classic" cloning method, and for... for many reasons, remains one of the most popular today. Restriction enzymes, which are naturally produced...

  8. Optogenetics Guide

    Type
    Guide
    ...and CIB1 450 LARIAT Inhibits protein function by reversibly sequestering targets into large, optically ...Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and cellular approaches for diversifying and extending...cells (frequently neurons) and their governing biomolecular processes. The tools and technologies developed...utilize light to detect, measure, and control molecular signals and cells in order to understand their... Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators In this overview...optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light through direct... trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic toolbox has been...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replication as it activates the necessary cellular machinery to produce large amounts of rAAV vectors. The E1 gene...a double-stranded circular episome which can concatemerize, producing high molecular weight structures...retinal disease, spinal muscular atrophy, hemophilia A and B, and Duchenne muscular dystrophy. Many clinical...cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production The three production...distributing widely throughout the organism to target large or diffused biological structures. Specifically,...visualize connections between neurons within the brain. Large-scale examples of mapping the brain through neuronal...researchers are used to detect, measure, and control molecular signals of cells (commonly neurons) in response...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common...First-generation First-generation lentiviral plasmids are largely defunct due to their lack of safety improvements...on different plasmids, the viral genome remains largely intact. First-generation plasmids include (Figure...cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production After cloning, ... shaping integration site preferences. A major cellular determinant is LEDGF (also known as PSIP1 or p75...cells Chimeric envelope genes have also gained popularity to further enhance both infectivity and specificity... dictate the target cell population and reduce cellular toxicity. Common Uses of Lentiviral Vectors Due...
Showing: 951 - 960 of 971 results