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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 981 - 990 of 1010 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction... select transformed cells containing plasmids differing in their resistance genes, as one does not need... give the antibiotic time to more fully absorb. During the incubation, transform DH5α E. coli cells by...

  2. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA You may also like... Recovering Plasmid DNA from Bacterial Culture Purifying ...enzyme digestion takes advantage of naturally occurring enzymes that cleave DNA at specific sequences....Buffer 3 µL 10x BSA (if recommended) x µL dH 2 O (to bring total volume to 30µL) The amount of restriction ...

  3. General Transfection

    Type
    Protocol
    ...hydroxide 0.22 μm polyethersulfone (PES) filter Syringes for filtering Reagent Preparation DMEM Complete: 10% ...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

  4. Lentivirus Production

    Type
    Protocol
    ... of powder in 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...DNA tube. Incubate the mixture 12–15 min at RT. During the incubation, add 10 mL of DMEM Complete to a... pellet any packaging cells that were collected during harvesting. Filter supernatant through a 0.45 μm...

  5. Using a Light Microscope Protocol

    Type
    Protocol
    ...knobs on the side of the microscope. You may be wondering why there are multiple objective lenses. Each ...with the lighting, use the coarse focus knob to bring your sample into focus. Once your sample is in focus...easily see your sample. Then use the focus knobs to bring the sample into focus. Pro-Tip As you increase your...

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...reference AAV of known titer, 100837-AAV1, and by measuring the titer of samples obtained from academic viral...transparent film. Centrifuge at 3,000 rpm for 2 min to bring the sample to the bottom of the tube. Run the following...amount of background signal that is most evident during initial PCR cycles. This background signal must...

  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... the sample is determined. Sharing speeds science. We believe that sharing the full details of our protocols...and ImageJ software. The sample is separated by denaturing polyacrylamide gel electrophoresis alongside ...

  8. Western Blot

    Type
    Protocol
    ...SDS-PAGE gel, and immunoblotting. Sharing speeds science. We believe that sharing the full details of our protocols...buffer is suitable for most proteins but more stringent buffers and a sonication step may be required ...

  10. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction... you have single colonies, you can proceed to Recovering Plasmid DNA or use the individual colonies for...
Showing: 981 - 990 of 1010 results