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We narrowed to 7 results for: AsCas12a

Showing: 1 - 7 of 7 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

  3. CRISPR Guide

    Type
    Collection
    ... many Cas12a orthologs, but the most common is AsCas12a (from Acidaminococcus sp. ). The popularity can...popular being EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR...Huang, A., & Doench, J. G. (2020). Optimization of AsCas12a for combinatorial genetic screens in human cells...

  4. CRISPR Guide

    Type
    Guide
    ... many Cas12a orthologs, but the most common is AsCas12a (from Acidaminococcus sp. ). The popularity can...popular being EnCas12a, an engineered version of AsCas12a that recognizes TYCV and TATV PAM sites. The DETECTR...Huang, A., & Doench, J. G. (2020). Optimization of AsCas12a for combinatorial genetic screens in human cells...

  6. Hot Plasmids - February 2022

    Type
    Blog Post
    ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...
Showing: 1 - 7 of 7 results