We narrowed to 296 results for: AsCas12a

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge