We narrowed to 157 results for: AsCas12a

1. AsCas12a

   Plasmid

   #188492

   Purpose

   Expresses FLAG tagged AsCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

   Plasmid

   #149722

   Purpose

   Lentiviral expression of crRNA with GFP and a neomycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDA174_EF1a_EnAsCas12a_BFP

   Plasmid

   #196724

   Purpose

   Sortable and selectable EnAsCas12a.

   Depositor

   Bernhard Schmierer

   Insert

   enASCas12a-HA-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS - HA and SV40 NLS

   Mutation

   E174R, S542R, K548R

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Topo_SpCas9.tracr_AsCas12a.DR

   Plasmid

   #155050

   Purpose

   TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
   

   Use

   CRISPR

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AsCas12a-DR-cr in PBCSK-

   Plasmid

   #126640

   Purpose

   Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   AsCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. enAsCas12a-mSA

   Plasmid

   #182954

   Purpose

   Express enAsCas12a-mSA

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   enAsCas12a-mSA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNS35 (multiAsCas12a piggyBac)

   Plasmid

   #217336

   Purpose

   piggyBac expression of multiAsCas12a

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJCC_076 AsCas12a R1226A

   Plasmid

   #171669

   Purpose

   For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a R1226A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginine 1226 to alanine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Promoter

    CMV IE94 promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-AsCas12a-2AeGFP

    Plasmid

    #123638

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    AsCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUPD2 AsCas12a (GB1438)

    Plasmid

    #160572

    Purpose

    Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid

    #217330

    Purpose

    Lentiviral expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid

    #217332

    Purpose

    piggyBac expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCH4 (denAsCas12a-KRAB lenti)

    Plasmid

    #217331

    Purpose

    Lentiviral expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCH68 (dAsCas12a-3xKRAB piggyBac)

    Plasmid

    #217334

    Purpose

    piggyBac expression of dAsCas12a-3xKRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    dAsCas12a
    

    Use

    CRISPR

    Tags

    nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid

    #217333

    Purpose

    piggyBac expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge