Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 7 results for: Dronpa-2

Clear keywords
Showing: 1 - 7 of 7 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...status of MEK1/2 was tracked at 5 minute intervals via Western blot. Phosphorylation of MEK1/2 peaks at just... phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. ...PubMed PMID: 28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey...activity by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...both activates and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less...light (see Table 2). The best known reversible PA-FP is the naturally occurring Dronpa protein (named after...fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2: Properties of Selected Reversible...for vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light,...chromophore, leading to a non-fluorescent state. Dronpa can be reversibly reactivated by exposure to UV...scientists used mutagenesis screens to find a version of Dronpa that doesn't become inactivated as quickly. Padron...monomeric, reversible PA-FP derived from mutating Dronpa (T59M / V60A / N94I / P141L / G155S / V157G / M159Y...

  3. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...interactions with photoactivatable fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity...living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide...a conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T...C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to ...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression Dronpa 503 518 400 (Off to On) 503 (On to Off) 81 40 min Monomer pcDuex2-Dronpa - Mammalian...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression... Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - ...Bacterial Expression Dronpa3 490 515 405 (Off to On) 490 (On to Off) 19 Monomer Dronpa3-N1 - Mammalian Expression...Expression Dronpa3-C1 - Mammalian Expression rsEGFP1 493 510 405 (Off to On) 491 (On to Off) 17 6.5 3 ...

  5. Optogenetics Guide

    Type
    Guide
    ... Name Description Peak excitation λ (nm) Dronpa Mutant Dronpa domains associate in the dark, inhibiting...influx into the cell when stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool,...protein interactions based on A. thaliana Cryptochrome 2 and CIB1 450 CRY2olig, CRY2clust CRY2 variants that...control of biological systems. Nat Rev Methods Primers, 2 , 55. https://doi.org/10.1038/s43586-022-00136-4 PMID... with single-spike temporal resolution. PLoS ONE, 2 (3), e299. https://doi.org/10.1371/journal.pone.0000299...comparative analysis of microbial opsins. Nat Methods, 9 (2), 159–172. https://doi.org/10.1038/nmeth.1808 PMID...illumination, allowing activity; Dronpa145N homotetramerizes, Dronpa145K and Dronpa145N dimerize with each other...

  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...the light-inducible heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 ...inactive, dead Cas9 protein (dCas9) fused to CIB1; and 2) the activator - the CRY2 photolyase homology region... fused to both the N- and C-termini of dCas9; and 2) CRY2FL-VP64, a fusion of full-length CRY2 and the...researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400-fold increase after 30 hr. The system...authors fragmented Cas9 into N-terminal (residues 2-713, N713) and C-terminal (residues 714-1368, C714...nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter the system...prior work, the Sato lab also showed that the paCas9-2 system could be spatially controlled and reversibly...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's, FTD Michael Davidson 57286 Dronpa-MAPTau-N-10 MAPT Dronpa CMV Parkinson's, FTD Michael Davidson...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS...196208 GB1-A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep...196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196211 GB1-A11(2-196, G175R...196212 GB1-A11(2-196, G189E)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ...deltaα2) CHMP2B HA CMV ALS Franz-Ulrich Hartl 232006 pCMV CHMP2B-L4D F5D-d97-106-3xHA (deltaα2/3 loop...TLS 1: hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C...
Showing: 1 - 7 of 7 results