We narrowed to 35 results for: Folding Reporter GFP

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

Overview of Addgene's viral production methods and quality control for lentivirus and AAV.

SunTag amplifies fluorescent signals by recruiting multiple copies of GFP. SunTag can help you turn up the brightness in your fluorescent imaging experiments.

Exciting new plasmids, viruses, and antibodies in the Addgene collection!

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

Opto-nanobodies (OptoNBs) are nanobodies that can be controlled by light. Learn how you can use OptoNBs to purify proteins or to modulate cell signaling.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- or dox-inducible systems.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.