CRISPR References and Information
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...PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: ...Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled libraries for large-scale functional...selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning... Cas9 D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ;...plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable ...