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We narrowed to 24 results for: halotag

Showing: 1 - 20 of 24 results

  1. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...protein of interest. For example, the Cohen lab used HaloTag for their "ticker tape" method for intracellular...tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular self-labeling...both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins...

  2. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...Exchangeable HaloTag Ligands HaloTag Reversible binding of fluorescent ligands to HaloTag PCV-Cas9, Cas9...Plasmid Collection HaloTag Collection of research tools from Promega, including many HaloTag plasmids Exchangeable... tube or in cells. There are also SNAP-tag and HaloTag modules, which can connect proteins to any kind...SNAP-tags or CLIP-tags Tetbow SNAP-tag, CLIP-tag, HaloTag Based on the Brainbow system, adds Tetracycline...

  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Adapted from Nabet et al., (2018).   HaloTag The HaloTag was initially developed by Promega as an ...straightforward system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the...proteolysis-targeting chimera (PROTAC) was developed for HaloTag. PROTACs are engineered molecules which target ...targeted to the proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave...involves imaging at some stage, then choosing a HaloTag could pay dividends down the road. It is difficult...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...interchangeable labeling using the HaloTag® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling...these self-labeling proteins are SNAP-tag® and HaloTag®. These tags consist of a genetically encoded enzyme...2017). These dyes were adapted into ligands for HaloTag® and are currently offered at Promega. Due to the...

  6. Hot Plasmids - February 2022

    Type
    Blog Post
    ...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

  9. Promega Plasmid Collection

    Type
    Collection
    ...protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in a new window) technology...fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking... tracking proteins using tags such as NanoLuc, HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids...such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other terms. Or search the collection...applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters...your hands, thanks to interchangeable ligands. HaloTags form a covalent bond with these synthetic chemical...even customize ligands for your specific purpose. HaloTag technology is optimized for various protein expression...

  16. Distribution to Industry

    Type
    Collection
    ...Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2,...

  17. Fluorescent Proteins: FRET

    Type
    Collection
    ... can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more about self-labeling tags...

  18. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876...pCS2+/N-Halo - Xenopus Expression pET51b-His-TEV-HaloTag7 - Bacterial Expression SNAP-Tag Benzylguanine ...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...chemigenetic calcium indicator for fluorescence using HaloTag ligands Bright and tunable far-red chemigenetic...chemigenetic calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator...
Showing: 1 - 20 of 24 results