...adjustment, spectral flow cytometry relies on “unmixing.” Unmixing uses a complex mathematical algorithm and... fluorophores from one another during spectral unmixing.     Figure 2: The full emission spectra...sample antibodies are from the same batch, to avoid unmixing issues from batch-to-batch variation. Tandem dyes...batch. This can present particular difficulty in unmixing if your sample is stained with a PE/Cy7 antibody...antibody binding, leading to discrepancies during unmixing. Additionally, it is critical to include unstained...cells within your reference group to ensure proper unmixing. This is because both compensation beads and cells...

... imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various colors. In fluorescent...each Nano-lantern, so Takai et al. used a linear unmixing algorithm to decode the individual colors. The...

... the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...

...spectra overlap, there are techniques, like spectral unmixing, which can be used to separate the fluorescent...

...additionally thank Monica Sentmanat, Victor Dillard, and Ayokunmi Ajetunmobi for their contributions to this work...