Skip to main content

We narrowed to 7 results for: nmi

Showing: 1 - 7 of 7 results
  1. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...adjustment, spectral flow cytometry relies on “unmixing.” Unmixing uses a complex mathematical algorithm and... fluorophores from one another during spectral unmixing.     Figure 2: The full emission spectra...sample antibodies are from the same batch, to avoid unmixing issues from batch-to-batch variation. Tandem dyes...batch. This can present particular difficulty in unmixing if your sample is stained with a PE/Cy7 antibody...antibody binding, leading to discrepancies during unmixing. Additionally, it is critical to include unstained...cells within your reference group to ensure proper unmixing. This is because both compensation beads and cells...
  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). This construct contains...      Figure 2: Crystal structures of CRBNmidi alone (A) and in a ternary complex with the multiple...2024). They were able to study and crystallize CRBNmidi on its own, with drug ligands, and in ternary ...ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design...
  3. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various colors. In fluorescent...each Nano-lantern, so Takai et al. used a linear unmixing algorithm to decode the individual colors. The...
  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...additionally thank Monica Sentmanat, Victor Dillard, and Ayokunmi Ajetunmobi for their contributions to this work...
Showing: 1 - 7 of 7 results