...for positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly...

... the bacteriophage lambda pir protein. This allows conjugation between a pir-containing donor and the ...enabled very efficient recombineering.     We were inspired by this work and sought to develop these negative...

...with R6Kγ origin; contains a mutant allele of the pir gene that maintains the plasmids at ~250 copies per...Am) robA1 creC510 hsdR514 endA recA1 uidA(∆MluI)::pir-116  Stbl2   JM109-derived. For cloning into and...nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with...

...below for additional considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived...

... 643/670 12.7 piRFP670-N1 miRFP670 642/670 12 pmiRFP670-N1 iRFP682 663/682 10.2 piRFP682-N1 iRFP702 673...iRFP713) 690/713 6.2 piRFP iRFP720 702/720 5.8 piRFP720-N1 iSplit 690/713 5.3 pPAS-E and pK-GAFm Photoactivatable...673/702 7.6 piRFP702-N1 miRFP703 673/703 8 pmiRFP703-N1 miRFP709 683/709 4 pmiRFP709-N1 iRFP (aka iRFP713...

...Bacterial Expression iRFP670 643 670 13 4 ~5 hr Dimer piRFP670-N1 - Mammalian Expression pBAD/HisB-iRFP670 - ...Bacterial Expression iRFP682 663 682 10 4.5 ~5hr Dimer piRFP682-N1 - Mammalian Expression pBAD/HisB-iRFP682 - ...Bacterial Expression iRFP702 673 702 8 4.5 ~5hr Dimer piRFP702-N1 - Mammalian Expression pBAD/HisB-iRFP702 - ...Expression iRFP (aka iRFP713) 690 713 6 4.5 2.8 hr Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial...Bacterial Expression iRFP720 702 720 6 4.5 ~5hr Dimer piRFP720-N1 - Mammalian Expression pBAD/HisB-iRFP720 - ...

...make singly or multiply phosphorylated proteins (Pirman et al., Nat Comm 2015). Improved phosphoprotein...coli permits programmable protein phosphorylation. Pirman NL, Barber KW, Ma NJ, Haimovich AD, Rogulina S,... Cell-Free Protein Synthesis. Oza JP, Aerni HR, Pirman NL, Rogulina S, ter Haar CM, Isaacs FJ, Rinehart...

...Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream...TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. . PCP4 - ...

... 7.3 for suspension cells. For adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each...200 μl. Wait 24 - 72 hr to allow cells to grow. Aspirate media. Add 50 μl DNA extraction solution per well...

...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To...the case of the stabilized promoters from Segall-Shapiro et al., they chose a TALE protein as the repressor...replication, the stabilized promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences...division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that the stabilized promoters were...during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under... proteins are needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a ...stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher ...

...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

...next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria..., it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is just the ...the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and...curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed...

...death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger domain containing...

...libraries) 165529 pOpen-DpnIR DpnI 165537 pOpen-SapIR SapI 165539 pOpen-Bst HF Bst HF 165548 pOpen-EcoRV...

...immune system to fight progressing cancers. It was inspiring to participate in the 2017 Cell Symposium on Cancer...Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed and...Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation... evade the immune system - COX inhibitors like aspirin may give our immune system the boost it needs to...

...members of the Shapiro lab at Caltech.   References and Resources References Piraner, D. I. et al. Going...That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs),...., Ho, G. H., Sawyer, D. P., Bourdeau, R. W. & Shapiro, M. G. Ultrasound imaging of gene expression in...

...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening...

...and ideas. Maintaining openness and looseness of spirit The second crucial element in harnessing the power...serendipity is maintaining an openness and looseness of spirit. Amidst the rigors of PhD studies and long days...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ...

...students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks to our guest blogger, Mary Tamer...

...tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor...proteins.  To infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric...outreach programs to encourage and embolden the spirit of scientific innovation amongst school children...