...for positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly...

... the bacteriophage lambda pir protein. This allows conjugation between a pir-containing donor and the ...enabled very efficient recombineering.     We were inspired by this work and sought to develop these negative...

...with R6Kγ origin; contains a mutant allele of the pir gene that maintains the plasmids at ~250 copies per...Am) robA1 creC510 hsdR514 endA recA1 uidA(∆MluI)::pir-116  Stbl2   JM109-derived. For cloning into and...nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with...

...below for additional considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived...

...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To...the case of the stabilized promoters from Segall-Shapiro et al., they chose a TALE protein as the repressor...replication, the stabilized promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences...division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that the stabilized promoters were...during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under... proteins are needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a ...stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher ...

...next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria..., it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is just the ...the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and...curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed...

...immune system to fight progressing cancers. It was inspiring to participate in the 2017 Cell Symposium on Cancer...Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed and...Sousa from the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation... evade the immune system - COX inhibitors like aspirin may give our immune system the boost it needs to...

...members of the Shapiro lab at Caltech.   References and Resources References Piraner, D. I. et al. Going...That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs),...., Ho, G. H., Sawyer, D. P., Bourdeau, R. W. & Shapiro, M. G. Ultrasound imaging of gene expression in...

...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an exciting way of listening...

...and ideas. Maintaining openness and looseness of spirit The second crucial element in harnessing the power...serendipity is maintaining an openness and looseness of spirit. Amidst the rigors of PhD studies and long days...and connections that emerge during moments of inspiration. Additionally, journaling in the morning has ...

...tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor...proteins.  To infinity, and beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric...outreach programs to encourage and embolden the spirit of scientific innovation amongst school children...

...students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks to our guest blogger, Mary Tamer...

...consequences While frustration and anxiety have inspired people to rally for science, others are concerned...science rallies scientists to political action, and inspires politicians and citizens to include science in...state in this map. Finally, the march is meant to inspire scientists to be more involved in their communities...

..., the optimal viral titer must be determined empirically (this is the case for any viral injection into...reach the axon terminal. Again, this must be empirically determined in your own system. Once you are confident...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

...the other hand some software tools make use of empirically derived and/or prior knowledge to inform on the... Hsu et al., 2013) used a combination of both empirical and approximate methods. The details of their ...“high mismatch limit”, “approximate design”, “empirical design”, “single-PAM design”, and “multi-PAM design...

... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to ...causing him some difficulty… To give him some inspiration, we’d love to see how you’re decorating your ...

...away all of our holiday decorations, our holiday spirit remains. We would like to thank everyone who participated...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

...The holidays may be over, but signs of holiday spirit still linger (despite the unseasonably warm weather... Miller lab annual #DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to...

...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ...