We narrowed to 662 results for: Clu

...journal club! What happens during journal club? There are many common ways to run the journal club. Some...competitive and relevant. Enter: the journal club.  A journal club is a recurring meeting amongst scientists...meetings. More recently, online journal clubs have become popular, including those based on Twitter. At my former...the facilitator(s) of the journal club? Where will the journal club take place? Preparing for the first...Before the first journal club, think about the meeting itself A journal club should aim to have many of...should almost universally include the questions “Would you attend this journal club again?” and “How would...of the journal club?” You also likely want to evaluate the “success” of the journal club. This can be a...

..., Musicophilia includes case studies of relevant neurological atypicalities, including a patient with ...Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a book club in the office...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...protagonist, some clever magic, and a satisfying conclusion? In this series, there is always another secret...in 2011 and is still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director...explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous conversations...book-loving Addgenies. I’d never participated in a book club before, but I had a few reasons for launching this...

...up cell clumps Success in a flow cytometry experiment depends on a variety of factors including sample ...instead of vortexing. Include proper controls To ensure accurate data analysis, include a complete set of ... by denaturing proteins and dissolving lipids including those of the cell membrane. In some cases, this...whole blood sample will contain a mix of cells including granulocytes, lymphocytes, and monocytes. Due ...different populations of immune cells express unique Clusters of Differentiation cell surface markers (CD) these... through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain cell...staining.  If you’re using antibodies for detection, include an unstained control with cells that went through...

...optogenetics toolbox with CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment...peptide. CRY2clust should prove a useful tool to further probe cellular function. The CRY2clust Plasmid...proteins in response to blue light. This tool, CRY2clust, was created by adding a 9-residue peptide to ...CRY2, although some studies have shown that this clustering only happens under certain conditions. With...With its additional 9 amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization...reversible upon termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics...sensitivity to light, and better distribution of clusters without accumulation in subcellular compartments...

... antibodies, include controls where each primary is omitted but all secondaries included to uncover secondary... shifts indicative of tau phosphorylation. By including the positive control, you will be able to confirm... addition to experimental samples (?). B, The inclusion of biological replicates such as multiple mice...Replicates can be technical or biological and are included to improve the precision and accuracy and therefore...repeated measurements of the same sample and are included to assess variability in the experimental protocol...: Microscopy-based antibody experiments should include a primary antibody specificity control, secondary...antibody. The secondary antibody control should be included with each experimental run (Burry 2011). Autofluorescence...

...particular interest this fall:  The BioBuilderClub BioBuilderClub engages high school teams around the...runoff from winter icing.BioBuilderClub is the only informal synthetic biology club of its kind, running...classes, some pick and choose a few lessons to include each year; others use BioBuilder as an afterschool... work with one of five BioBuilder kits — which include lab materials and reagents such as droppers, centrifuge...biology.Launching with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group...completion, from pitch to prototype. Last year’s BioBuilderClub teams’ biological engineering designs and ideas... year from remote scientist mentors and the BioBuilderClub community. Registration is open through October...

...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...6). Many of the new administration’s actions, including taking down the climate change page on whitehouse.gov... consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that...with one unifying interest (the advancement and inclusion of science) they come from many different places...see and understand data themselves can come to conclusions themselves instead of depending on scientists...that ‘facts’ are subjective or alternative. Inclusion of data informing policy specifically related ...the most profound impact (15). Diversity and inclusion in science, technology, engineering and mathematics...

... the Chi-Bin Chien lab (Kwan et al., 2007) and includes four promoters, six fluorophores with nonoverlapping...promoters for cell types directly linked to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3...CyOFP1), some of the exciting properties of mCyRFP1 include a redshifted-emission spectrum compared to that...The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1 expression... was initially limited by off-target effects, including the conversion of other cytosines near the target...knockouts replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes... cassette via Cre recombination. Each knockout includes unique barcodes and universal priming sites to...

...you have many career options available to you including careers in academia, industry, non-profit, and... to maintaining a status in the United States including green cards that are well-tailored to meet the...specialized knowledge in a field of human endeavor including but not limited to biotechnology, chemistry, architecture...publications, and plan for future activities; including the interest of the greater scientific field, ...entities or individuals. A successful NIW case should include evidence such as proof of US government grants ...fact is best proved through objective criteria including awards, media attention, citations to your work... discuss your work. Participating as a judge (including as a peer reviewer for journals, acting as a journal...

... an array of post-translational modifications including protein folding, formation of disulfide bridges...for protein production and purification. These include mammalian, insect, bacterial, plant, yeast and ...summarize some of the more common expression systems including their advantages and caveats to keep in mind before...Some drawbacks of insect cell expression systems include that virus production can be quite time consuming...produced by bacteria become insoluble, forming inclusion bodies that are difficult to extract without harsh...requirements as mammalian cell expression systems, including the majority of complex post-translational modifications...been used to express a wide range of proteins including antibodies and pharmaceuticals, specifically interleukins...

...2022 AAV.MaCPNS2 PNS, including ENS (mice) PNS (rats) CNS; PNS, including ENS (adult marmosets, infant...vector, which determine the vector’s tropism, including its ability to cross barriers like the BBB.)  ...application is dependent on a number of factors, including species, strain, age, sex, area of interest, and... with unique tropisms are now available. These include variants with broad CNS or PNS transduction, cell...expression control. (c) Viral delivery methods include focused ultrasound (FUS), intracorporeal (IC), ...expression or activity can be achieved using switches, including riboswitches and optogenetic and chemogenetic ...more effective than AAV9 in many other strains, including FVB/NCrl, 129S1/SvImJ, DBA/2J, and C57BL/6J, the...

...adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright...however the nanobody proteins tend to get stuck in inclusion bodies. Takayuki Iwaki, Kimiko Hara, and Kazuo...described a simple method which alleviates the inclusion body problem and makes nanobody production in ...inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify...Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases... uses CRISPRi to control gene expression. This includes a CRISPRi-based synthetic oscillator, bistable...of norepinephrine. These norepinephrine sensors include GRAB_NE1h (high affinity) and GRAB_NE1m (medium...

...not worried about including irrelevant events in my analysis, as these will get excluded through further...gate on single cells and exclude any events where multiple cells were clumped together. (B) Instead of...gate to identify cells, the single cell gate to exclude duplicate events, and the Live/Dead gate to focus...your cells of interest, you will likely need to exclude all duplicate events (two cells stuck together)...gated my single lymphocyte cells, I now want to exclude all dead cells from my analysis. For this measurement...Lastly, you learned which gates should always be included in your analysis, regardless of your experiment...Sharpe at Harvard Medical School. His projects include investigating negative regulators of T cells and...

.... Conclusion: Always, always, write a brief conclusion of your analysis, even if your conclusion is ...is “this approach is not ideal because…” Including a conclusion for each analysis or task will keep you... approaches to analyze large data sets (myself included) and we are faced with finding the best system...begin, briefly state the goal of the analysis, including background on other analyses that have inspired...where you also record your goal, approach, and conclusions (see above). Keep original files in a separate...

...The CRISPaint toolkit includes several donor plasmids for protein tagging, including luciferase (NanoLuc...regulatory sequences, including protein enhancers and repressors, can be included in the repair template...have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear...accepts the homology arm repair template. SapTrap includes a prebuilt donor plasmid library containing several... tagging genomic loci in other model systems, including mammalian cells. CRISPaint (CRISPR-assisted ..., thanks to the modular arrangement of vectors included in tagging systems like SapTrap, scientists will...

...(Figure 4), our catalog includes vectors in a variety of serotypes, including the retrograde AAVrg and...and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors 101 2nd edition...system can be affected by any number of factors, including the age of the model organism, injection site,...resources for producing vectors from plasmids — including the protocols we use in our lab — being able to...engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category... for vectors in the “controls” category, which includes things like viral vectors that deliver GFP. ...community. Thoughtfully prioritizing which preps to include in our service allows us to make ready-to-use viral...

...choose, it is essential to include proper controls. As a positive control, include a sample that is known ...working and should always be included.  For a negative control, include a sample that does not contain... the target!) and use them in numerous fields including research and development, diagnostics, drug discovery...background staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing... protein, especially one with tags, be sure to include a sample that expresses the endogenous protein,...

... identifying lineage markers, like many of the cluster of differentiation (CD) proteins found on immune...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies...fluorescence-activated cell sorting (FACS) enables you to include further (non-lineage) markers alongside, e.g., ...unlabeled cells of interest while the rest can be excluded, or “dumped”. From left to right: B cell, CD4+...as proteins of the secretory pathway. Those can include cytokines and chemokines; enzymes like kinases,...solution can be the same). Examples of such targets include transcription factors, histones, and DNA repair...still be easily gated on during the analysis. Conclusion Congratulations! You made it through this blog...

...Whenever possible, include positive and negative controls. Positive controls typically include cell lines or...several variations of the protein transfer step including wet, semi-dry, and dry but all follow the same... Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot ...data sheet thoroughly. Vendors will frequently include suggestions for working concentrations, blocking...blocking buffers, and incubation times. If the vendor includes publications that cited the antibody, consider...It is important to note, however, that you must include a loading control when assessing protein abundance...western blot. When planning a western, take care to include the proper positive and negative controls, choose...

... software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be detected...also eliminates many of the extraneous ORFs that cluttered our old plasmid maps. Clearly Annotated Features...greater than our previous mapping software and now includes Type IIS restriction enzymes that are commonly...tooltip information and sequence analysis tabs including: Maps This is the first tab you’ll land on...enzymes that cut the given sequence. The table includes enzyme names, the locations where the enzymes ...features detected in the plasmid sequence. The table includes feature names, locations, sizes, colors used to...detected in a given nucleotide sequence. The table includes primer names, sequences, binding site locations...

...intentionally excluded any libraries from the software, and my hope is to continue including other libraries...strategies beyond SpCas9, or species not currently included in Green Listed.  Provide the list of genes ...Input” box. Choose from different “Options”, including adding adapter sequences for cloning purposes.... output folder containing several files. These include: Output: A text file containing full information...information about the suggested gRNAs to use, including all information provided in the original reference library...Output_Compact: A text file that describes which genes were included, and how many gRNAs were identified for each gene... gRNA, as well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all...

...collection of plasmids for coronavirus research, including a handful of plasmids that have been in the repository... plasmids used for this study at Addgene which include a C-terminal deletion in the spike protein to improve...Plasmids deposited from Alejandro Balazs’s lab includes those for generating pseudovirus and those encoding...the widely used qPCR and antigen tests.  Labs including Jennifer Doudna’s lab and Feng Zheng’s lab had...for detection have been made openly available including those from Andrea Pauli and Julius Brennecke. ...19 diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase ...untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection...

...results, conclusions, and acknowledgements sections. Some other additions you might wish to include are a...navigable and efficient; your questions, data, and conclusions should be clearly identified and interpreted ...smaller than in other poster sections, so you can include a lot more. Font size: Making your poster readable...equally-sized columns, where the introduction, methods, conclusions, and acknowledgements sections are inserted. ...the central region, and a scannable barcode is included that links readers to more information if they...background but want to add a bit more “flash,” you can include a subtle gradient (as is shown in all of our illustrations...

...bacteria, including many natural plasmids. By contrast, shuttle vectors are lab tools, and they include both... vector designed for unrelated bacteria should include oris for both target species. Fun fact! Plant vectors... genome. One option for episomal vectors is to include viral oris to drive amplification, which helps ... affects all kingdoms of life. Of course, that includes you. Besides the risk to the researcher, Zeocin...approach in a mammalian cell experiment should still include an antibiotic resistance gene for growth in bacteria... shuttle between prokaryotes and eukaryotes. Conclusion I still think it’s pretty incredible that my ...

... open access publisher has required authors to include a note as to where readers may locate data supporting...information) for assessment of veracity of the conclusions drawn. The main difference with the PLOS policy...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...information is made available to everyone at once, including the generators. This practice doesn’t appear to...scientists are, however, privileged in having exclusive access to data and enjoy reasonable time to make...The fact that so few authors now query what to include suggests pragmatic adoption (although it might ...access to data is important, what data should be included? We should recognize that storing data in a sharable...

... allows it to be used in more E. coli strains including the popular BL21(DE3) (Li et al., 2021). This ...original pCas/pTargetF but has many other advantages including shorter plasmid curing time. pCRISPomyces for ...Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces.... INTEGRATE consists of four major components including (1) a CRISPR RNA, (2) four proteins forming the...essential genes in a wide variety of bacteria including in pooled, genome-wide, formats. Interested in...proteins involved in the activation of transcription including transcription factors, phage proteins, and RNAP...Adding complexity, they found that they could also include a CRISPRi system for multiplexed modulation. CRISPRa...

...sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats...chromosome encodes a tracrRNA (in some systems including Cas9), Cas proteins, and a CRISPR array. The CRISPR...is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria, these ...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-...RNAs” are transcribed from the CRISPR array and include a spacer and a repeat sequence. The spacer sequence...mismatches can be tolerated outside this region. Conclusion Ready for some more details? Check out the Addgene...

...data, you can even include your own data in a ReFigure. For example, you might include a confirmatory image...cofounder of ReFigure. Reading and exploration including replications and experiments resulting in “negative...also eLife, PLOS and Figshare). Some use cases include: Cataloging a reagent’s performance as in this...remain hidden from research papers as they are not included in traditional publications. By submitting your... currently supported by ReFigure beta) you can include it in a ReFigure. ReFigures are good for you and...

...Addgene is building our antibody resource, which includes recombinant antibodies, educational materials,...California Davis School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging...disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies and...antigen, and performance in various applications are included in the full webpage. Educational Resources...discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence, conjugates...over time. Through our incredible community, including your support, we have enabled scientists to easily...

...many roles that actively require these skills, including: Science Reporter Scientific Sales Epidemiologist...Careers where you’ll actively use these skills include: Science Reporter Scientific Illustrator Research...outdoor skills are needed in a variety of roles, including: Livestock Breeder Scientific Illustrator Plant...etc) are highly desirable in a range of roles, including: Science Reporter Technical Writer Statistician...position. Questions you’ll want to find answers to include: Will you be sitting at a desk all day or be on...to the content in your Resume and Cover Letter. Include any relevant job specific ‘buzz words’ to ensure...

...from the company LinkedIn page. This can include news, inclusion, culture and connections. Start your interview... is within a few days after you’ve met. Always include a note about where you met IRL (in real life) and... a connection request to any of these people, including and especially the hiring manager. This is considered.... Follow all the rules of good resume writing. Include examples of concrete accomplishments in both the...the technical and “plus skills” areas. Include a professional picture. It doesn’t have to be a headshot...

...results that ultimately builds up to an overall conclusion(s). In some articles, the data are presented ...to build a case for a certain model or overall conclusion. In others, the experiments are geared towards...possibilities until the results focus in on a particular conclusion. Many papers aren’t always written this way and...the results to see if it leads you to the same conclusion. Personally, I like papers that can end with ...are appreciated) to visually sum up all of the conclusions. In any case, the reader should give a critical... the relation to how they are supporting the conclusions. Look at the figures and write down your own ...

...genes that regulate a wide variety of phenotypes, including but not limited to cell growth and drug resistance...activate Ras are found in one third of all cancers, including a high percentage (up to 95%) of pancreatic cancers...crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to...provided in both open (no stop codon) and closed (including a stop codon) formats. The collection is compatible...collection of over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters... a new method for generating protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016...key host for the production of their products, including fermented beer and wine, fuels, pharmaceutical...

...experimental processes, results, and subsequent conclusions. Being vigilant about these errors is an important...easy to share. However, the cloning process — including the propagation of plasmids in bacteria — has ...and money on optimizing experiments, to false conclusions.  Figure 1. Potential points where DNA..., indicated by exclamation marks. Error points include places where DNA is being created, such as PCR ...believe may affect the function of the plasmid. This includes point mutations, frame shifts, missing elements...require additional attention during analysis. These include topics such as: locations in a plasmid that are...

...genetics. In these conversations, pgEd strives to be inclusive of all voices, regardless of socioeconomic or .... We do this through a variety of activities, including creating educational materials and lesson plans...to hamper efforts to engage these communities, including those by researchers hoping to increase community...leadership and communication experience. These included taking extra coursework and attending seminars...of organizations in public engagement efforts, including giving public lectures, writing science articles...our experiences in a variety of other settings, including “conventional” academic venues such as scientific...

...of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a glossary at the end if you...reaction performed by CBEs. However, most CBEs include an inhibitor of base excision repair (BER), the...Multiple labs tried to identify beneficial factors to include in even better cytosine transversion editors. New...proteins had additive effects when multiple were included in the CGBE. They also developed a machine learning...cellular processes that would cause DSBs, and its inclusion significantly reduced indels generated by CGBEs...complementary region of the non-target strand is excluded as ssDNA. DSB Double-Stranded Break A cut to...

..., and include information on tested antibodies against the target. Helpfully, this also includes clone...antibody for flow cytometry experiments. This will include a discussion of the Human Leucocyte Differentiation...approach Genetic knockout-based validation can conclusively prove the ability of an antibody to detect the...separation and reduce any background staining. This can include using: different blocking reagents; titration of...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown is...molecules expressed on human blood leukocytes, including antibodies against these markers. The 371 CD markers...

...sites are the most inclusive markers of regulatory regions in the genome, including enhancers, promoters...anywhere in the genome. Aneesh's research interests include proteomics, machine learning, and visualization...maps to find all coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might...project, find enhancers in embryonic stem cells, and conclude with a targeted CRISPR screen that can disrupt...screens for enhancers, check out the Appendix. Conclusion: Shedding light on dark matter Precise knowledge...

...chimeric 5'LTR that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a chimeric 5'...plasmids created for a variety of applications including cDNA expression, shRNA-mediated knockdown, Tet...lentiviral packaging vector, a transfer vector including the gene of interest, and the plasmid coding for...is used; several common laboratory cell lines including 293 were immortalized with adenoviral protein ... stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral vectors...

... activation (CRISPRa) tools for neuroscience, including transgenic mice, neuron-optimized viral vectors... large size of Cas9 activators, which largely precludes delivery by adeno-associated vectors (AAV). This... express Cre under neuron-specific promoters, including calmodulin-dependent kinase II alpha (aCaMKII;... in primary rat neurons over other promoters, including ubiquitous ones such as EF-1α and CAG. ... the differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes, provided...applications for which CRISPRa is well-suited, including studying mRNA dosing effects, cellular reprogramming...