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  1. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... process. Industry interviews, however, were more structured. I met with several individual and/or small...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...search for “questions to ask an interviewer,” you can find lists that have lots of general questions about ...

  3. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...After an inducer binds to the activator protein, the activator protein can bind to the promoter, turning...repressible promoters. How are inducible promoters regulated? Inducible promoters can be regulated by ...Positive inducible In the OFF state, the promoter is inactive because the activator protein cannot bind. After...protein actively prevents transcription. Once an inducer binds the repressor protein, the repressor protein...factors that can lead to the induction of a promoter. Below, you’ll find a short description of these ...Chemically inducible promoters Chemically regulated promoters are among the most common inducible promoters...simplified lac inducible system is found in many bacterial expression vectors. Negative inducible promoter ...

  4. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...consistently welcoming. This bears repeating. Individuals that work in science policy are a treasure--they...do to creatively connect seemingly disparate individuals and ideas toward solving one problem. It often...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...possibilities are endless leading to many ways for you to find your own niche.  Action Items:  Explore this career...school or a postdoc. But some are also open to individuals from mid- to late-career backgrounds. In fact...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Doudna JA (2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science... a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can promiscuously...activated” Cas13 produces a quantifiable signal that indicates the presence of your targeted nucleic acid. To...activated by Cas13 ssRNA cleavage byproducts. Thus, binding of Cas13 to the programmed region of interest would...providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of...selectivity and sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...describe in more detail below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main...structurally separates the two components of the indicator, which can make the kinetics of voltage sensing...Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room...

  7. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...ΔF/F0 heatmaps. White insets indicate surface expression of the indicators over white dashed lines. Scale...Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...They can, however, be studied with the nLight indicators. Speed One of the major advantages of this next..., ten pulses. This is in line with the general findings that red fluorescent proteins tend to be less ...to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ... It is worth doing some some serious pre-work to find a lab that will be a good fit for you and your career... suggestions on planning for future success by finding and joining your dream lab, watch the webinar "...Executive Director, Joanne Kamens.   The goal: Finding your dream lab While none of us will feel completely...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...library allows researchers to reconstruct the full individual sequence. Traditional NGS workflows are limited...costly library prep, as each sample requires an individual library. The plexWell™ workflow is a sequential...continuing to process 100s to 1000s of samples individually, plexWell™ reduces every 96 well plate to a ...often arises from the fact that large numbers of individual input DNA samples (e.g. a collection of 96 plasmids... prep methods because the yield or quality of individual libraries can be influenced by the quantity or...usually include a separate normalization step where individual input DNA samples are brought in line with each...normalization, it is also often the case that individual libraries prepared from multiple samples will...

  10. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    Published
    March 16, 2016, 5:30 p.m.
    ...perfect for my next experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help... we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository? ...name of the plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone...

  11. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... understand. I did, however, find it very hard to speak with individuals who had very strong beliefs in...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...roles in the field require experience. Working in industry: Microbiology On returning from Ghana at the beginning...personal bank loan. I saved money from my role in Industry and took evening work to help cover the costs....growing. These factors left me optimistic that I would find a genetics related role soon after finishing my ...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...many find it helpful to obtain support and ideas from those who seem to be managing. Being reminded of ...time. #1 Thou shalt find enjoyment in your work If you are happy at work, you will find balance. If you enjoy... you are not happy at work, you probably need to find a new job. If you are miserable at home or work,...better. #8 Thou shalt enthusiastically foster independence Your partner can make his or her own dinner ...assignments, the better. In my opinion, a level of independence is better for them anyway. You will worry less...the time my eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin...

  13. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...other great tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One...describe some of the many ways you can approach finding someone to give you advice and guidance. Second... the more potential mentors you will identify. Finding potential mentors is one of the many reasons to...group mentoring program in many of its chapters. Find out what other groups have going on locally. Your...good coach can make a big difference, if you can find one. Create your own peer mentoring circle. This..., “This was really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships...

  14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-... your model system? Turns out that with light-inducible photosensitizers and a quick zap of the proper...case). The fluorogen is not fluorescent until it binds its protein partner thus forming the full FAP-TAP...FAP-TAP complex where dL5** is the FAP. It’s job is to bind MG-2I, a photosensitizer fluorogenic dye.  MG-2I...MG-2I-dL5** He et al tested out light-induced cell ablation with MG-2I-dL5** in two different experimental...present 96 hours post treatment (see figure 2). Induced cell death was confirmed by TUNEL staining.  The...ablation efficiency, but dL5** phototoxocity is induced when expressed on the cell surface, in the cytosol...

  15. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...beautiful Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge...Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in...badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge to be taken...them in experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical...are browsing article abstracts you should see the “Find Plasmids” box on papers that have deposited plasmids... find plasmids...

  16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...! The starting point: Ligation-independent cloning Ligation-independent cloning (LIC) was first developed... personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does...specially designed plasmids. Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received...His new method, named sequence- and ligation-independent cloning (SLIC), eliminated many of LIC’s constraints...PubMed. SLIC: a method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol....guide to SLIC Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing...

  17. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...). Best uses Finding papers frequently cited by others. This is a great hack for finding relevant papers...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding... finding relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight...highlight four tools for finding journal articles related to your actual interests while also staying on top ...PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996, but is getting...be summarized in 280 characters! Using Twitter to find papers  You’ll need to start following relevant ...accounts in order to turn Twitter into a paper-finding-machine. There are four general types of accounts...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...same information in our search indices in order to support different kinds of matches: exact words, partial...Fortunately, we’ve just made it easier for you to find what you need quickly.  Through the years, we have... a result, resources on the website were hard to find and many remained undiscovered.  To remedy this,...Now, let’s take a look at the least visible part behind these changes: the backend technology that powers...powers the new website search. Tackling findability from multiple angles    We assembled a cross-team team...needed to improve more than that: the general “findability” of the information on the site. Different users...users have different strategies for finding what they need, and we wanted to support all of them — searching...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...MIcrobiology Pages The problem: Host binding proteins are hard to find Sequencing and cloning phage DNA can...evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition factors of...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage..., phages represent a reservoir of carbohydrate binding proteins waiting to be explored and exploited. ...phage genome to be probed for proteins with host-binding activity without the need for genome sequencing...sequenced genome that encode for functional cell-binding proteins in the (all-too-common) event that homology-based...coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished using a...

  20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...referrals. There are bad coaches out there, so finding a good one that is a style match for your needs... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...Resume That Gets Seen  Guide Your PhD with An Individual Development Plan Learn a Little about The Academic...Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

  21. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...Podcast.      Additional Resources from Addgene Find more management resources in our Mentoring for Scientists...

  22. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ...and individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide...all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) and guide-only ...in the first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes...themselves to screening in a pooled setting. The individual plasmids will also be useful for imaging or gene...expression assays necessitating the screening of individual plasmids separately. Additionally, this set can... can be used for cherry-picking individual gRNAs that score as hits in pooled screens, facilitating more...follow-up. We hope the biological community will find these reagents to be useful. Happy screening! Many...

  23. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...has been referenced in other publications, you'll find a link to the list of citing articles under the ...article list you can click on the PubMed link to find the article abstract and more. We've also included...into a previously deposited vector - then you'll find the plasmids listed below the citation. An example...Barcoding cells using cell-surface programmable DNA-binding domains" publication.  If you want to see this...identify relevant protocols and experimental data Find other related plasmids in the "Associated Plasmids... Happy researching! If you need any assistance finding plasmids on Addgene's website or would like to ...

  24. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...of the juicy and important details that you won’t find in the final publication and we have already collected... AAV Data Hub grows, we hope to mine the data to find patterns that we can share with you about promoters...

  25. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...University became openly available. Find plasmids available to industry scientists To make plasmid sharing...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...sharing more open for academic and industry scientists, we’ve worked with many scientists, tech transfer offices...

  26. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized...thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9...

  27. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral... sentences that I found hard to understand or individual words that are not appropriate for the given ... attention to details and use a checklist for individual sections of the paper (see figures). After finishing...speaking to a colleague in the office next door. So be kind, helpful, and thorough in reviewing the paper of...Magdalena Julkowska did her Ph.D. on salt-stress induced responses in Root System Architecture of Arabidopsis...Prof. Mark Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, ... Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990...

  28. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...you’ll find Addgene’s newly launched search engine results much better. Here, I wanted to find a specific...plasmids with more than the indicated number of requests Availability to Industry: Restrict search to plasmids...Finding the best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids...plasmids from around the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad...articles. From paper to plasmid Authors regularly indicate that their plasmids are available from Addgene... show the range of different terms we can use to find materials from this publication. Searching for the... the title of a paper works You can also find the associated plasmids through the article’s PubMed ID...

  29. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab...reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to...Addgene’s stem cell page. On this page, you can also find a table with a list of methods and the species they... hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. ... cases where the objective is to fix a disease-inducing genetic mutation in a patient, trying to transdifferentiate...PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...

  30. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...disadvantages led to the creation of induced pluripotent stem cells or iPSCs. Induced pluripotent stem cells iPSCs...Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ...works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely...: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic...PubMed PMID: 16904174. 9. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...72. PubMed PMID: 18035408. 10. Yu, J., et al., Induced pluripotent stem cell lines derived from human ...Medvedev, S.P., A.I. Shevchenko, and S.M. Zakian, Induced Pluripotent Stem Cells: Problems and Advantages...

  31. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...fused to a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin...it’s briefer time scale and its inducible and reversible nature. Find the LADL plasmids at Addgene Kim...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins... the AID technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova ...specific PAM (protospacer adjacent motif) in order to bind the target sites for editing. PAM sequences can ...they also fused the SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated...

  32. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress... from. Let’s take a look at some of the stories behind the names. We also have many plasmids in our repository...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however...terrible fate when she pricked her finger on the spindle of a spinning wheel but also a biological transposon...vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the name but some of our...properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval...Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene...

  33. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast... genetically encoded voltage indicators. To expand the voltage indicator toolbox, Ed Boyden’s lab developed...small scaffold proteins to identify novel binding proteins that bind to either the light- and dark- states...cPYP), and plasmids that encode the protein binders (which bind to either the light- or dark- state of the..., because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools ...for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis JM, et al. ...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...

  34. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...the ribosome binding site is inaccessible. When the target RNA is present, however, it binds to the switch...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic ...an option, including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol...switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be expressed...display and deep sequencing, revealing that they bind to a much larger subset of protein motifs than previously...biophysical study involving protein stability or binding experiments. Potential experiments that one might...

  35. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...publically available through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869...Toolkit Many industries, such as the food and beverage industry, the bioethanol industry, and the pharmaceutical... make it easier for you to find and use the plasmids you need. You can find all the hot plasmids from ...Nile Virus (WNV) induced cell death. The Wu lab library consists of 77,406 individual gRNAs targeting ...optimizations are required for each individual target species. Indeed, in order to use MAGE, the λ Red recombinase...for genetic optimization of industrial yeast strains. However, industrial yeast strains are more difficult...Targets of RNA-binding proteins identified by editing Identifying targets of RNA-binding proteins (RBPs...

  36. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...FKBP rapamycin binding domain of mTor (Cas9(N)-FRB-NES) resulting in a rapamycin-inducible Cas9 for genome...Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to... not show large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold...throughput binding assays, to engineer photoswitchable dimers which show over a 50 fold change in binding affinity... coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding sites to tether...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...

  37. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...ON. When a repressor binds the activator protein, the activator protein cannot bind the promoter sequence... cannot bind the promoter sequence and transcription is ON. Once a co-repressor protein binds the repressor...derivatives is added, it binds tTA, resulting in a confirmation change that prevents binding to the promoter and...cumate is added, it binds cTA, resulting in a confirmation change that prevents binding to the promoter and.... As ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1...system). The GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore...manner, with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4...

  38. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...interactions. Next comes the binding step where the target antigen binds to antigen-specific antibodies...conjugated antibody binds to antigen coated on the multiwell plate. In an indirect ELISA a primary antibody...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to the primary...antibody that binds to the antigen in a sample and then a second detection antibody binds. The detection...less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA the reporter...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of ... easy to find,  it is often easier for labs to source appropriate antibodies for an indirect ELISA than...

  39. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...dark-induced dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table...controlled binding, but with opposite effects: insertion into loop 1 caused dark-induced binding while loop...OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue light inducible and dark-inducible...OptoNB can bind, it co-localizes with its target at the cell membrane and when the OptoNB can’t bind, it localizes...loop 6 insertion caused blue-light induced binding. The lab used this general design scheme to generate...the effect of AsLOV2 insertion site on light-induced binding.  Light controlled protein purification with... path to light-controlled protein binding, they probably don’t bind your favorite protein yet. More OptoNBs...

  40. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...While a primary antibody binds to the protein of interest, a secondary antibody binds to the primary antibody...primary antibody, due to increased binding affinity, many indirect antibody protocols are designed to ...anti-mouse secondary will bind to all the primary antibodies indiscriminately, giving them the same output...antibody with a signaling molecule, the antibody binds to a protein, and then voila! Wherever your protein...protein of interest, it may be time to look at the indirect approach - which means it’s time to talk about...antibody. In the indirect approach, the signaling molecule is conjugated to the secondary antibody instead...antibody. Since multiple secondary antibodies can bind to a single primary antibody, this approach can ...
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