...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding additional...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit...us know what you would like to see available to industry scientists. Please note this does not guarantee...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...of tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...system induction. Investigate whether or not your serum is tetracycline-free. Tetracycline-inducible Empty...Empty Backbones Find a construct that will allow you to insert and induce your gene of interest. ID Plasmid...absence of binding of tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression...vectors for tet-inducible gene or miRNA expression rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains...Overview of tetracycline regulated inducible transcriptional activation or repression Plasmid...

... TALengineering Reagents TALE Repeat Plasmids Individual REAL TALE Repeat Plasmids You may also like.....longer arrays. These 28 plasmids are available individually or as a set as part of the Joung Lab REAL Assembly...

...: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD) a T ranscriptional R epressor D omain...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T...iPSC (Link opens in a new window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG...a new window) Wendy Gold A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy...a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts Unpublished (Link opens in a new window) Wendy... opens in a new window) RettBase - MECP2 mutation database (Link opens in a new window) OMIM Rett Syndrome...in a new window) OMIM MECP2 - MECP2 curated literature information (Link opens in a new window) UniProt...

... an industry setting. Scientific community and mentorship: As an industry postdoc, you might find yourself...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...

...After an inducer binds to the activator protein, the activator protein can bind to the promoter, turning...repressible promoters. How are inducible promoters regulated? Inducible promoters can be regulated by ...Positive inducible In the OFF state, the promoter is inactive because the activator protein cannot bind. After...protein actively prevents transcription. Once an inducer binds the repressor protein, the repressor protein...factors that can lead to the induction of a promoter. Below, you’ll find a short description of these ...Chemically inducible promoters Chemically regulated promoters are among the most common inducible promoters...simplified lac inducible system is found in many bacterial expression vectors. Negative inducible promoter ...

... process. Industry interviews, however, were more structured. I met with several individual and/or small...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...search for “questions to ask an interviewer,” you can find lists that have lots of general questions about ...

...consistently welcoming. This bears repeating. Individuals that work in science policy are a treasure--they...do to creatively connect seemingly disparate individuals and ideas toward solving one problem. It often...organizations, universities, professional societies, and industry through convening activities, formal lobbying,...possibilities are endless leading to many ways for you to find your own niche.  Action Items:  Explore this career...school or a postdoc. But some are also open to individuals from mid- to late-career backgrounds. In fact...and local governments, professional societies, industry, and beyond!  Learn and develop valuable science...

...Doudna JA (2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science... a virus or pathogen. Once Cas13 recognizes and binds to the programmed sequence, it can promiscuously...activated” Cas13 produces a quantifiable signal that indicates the presence of your targeted nucleic acid. To...activated by Cas13 ssRNA cleavage byproducts. Thus, binding of Cas13 to the programmed region of interest would...providing reliable results in as little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of...selectivity and sensitivity from patient samples. Find the DETECTR plasmids here! Applications of DETECTR...

...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...describe in more detail below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main...structurally separates the two components of the indicator, which can make the kinetics of voltage sensing...Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room...

...ΔF/F0 heatmaps. White insets indicate surface expression of the indicators over white dashed lines. Scale...Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...They can, however, be studied with the nLight indicators. Speed One of the major advantages of this next..., ten pulses. This is in line with the general findings that red fluorescent proteins tend to be less ...to be accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and...

... It is worth doing some some serious pre-work to find a lab that will be a good fit for you and your career... suggestions on planning for future success by finding and joining your dream lab, watch the webinar "...Executive Director, Joanne Kamens.   The goal: Finding your dream lab While none of us will feel completely...

...library allows researchers to reconstruct the full individual sequence. Traditional NGS workflows are limited...costly library prep, as each sample requires an individual library. The plexWell™ workflow is a sequential...continuing to process 100s to 1000s of samples individually, plexWell™ reduces every 96 well plate to a ...often arises from the fact that large numbers of individual input DNA samples (e.g. a collection of 96 plasmids... prep methods because the yield or quality of individual libraries can be influenced by the quantity or...usually include a separate normalization step where individual input DNA samples are brought in line with each...normalization, it is also often the case that individual libraries prepared from multiple samples will...

...perfect for my next experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help... we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository? ...name of the plasmid and a publication where we can find the plasmid. While we cannot guarantee that everyone...

... understand. I did, however, find it very hard to speak with individuals who had very strong beliefs in...applied for a role in Industry. I wanted to get "a real job" and roles in Industry do tend to pay better... (GLP) (important buzzwords in industry) as well as skills in industry positions. There were, however... a little different. Industry and academic jobs do exist, but working in industry just wasn't for me, ...roles in the field require experience. Working in industry: Microbiology On returning from Ghana at the beginning...personal bank loan. I saved money from my role in Industry and took evening work to help cover the costs....growing. These factors left me optimistic that I would find a genetics related role soon after finishing my ...

...many find it helpful to obtain support and ideas from those who seem to be managing. Being reminded of ...time. #1 Thou shalt find enjoyment in your work If you are happy at work, you will find balance. If you enjoy... you are not happy at work, you probably need to find a new job. If you are miserable at home or work,...better. #8 Thou shalt enthusiastically foster independence Your partner can make his or her own dinner ...assignments, the better. In my opinion, a level of independence is better for them anyway. You will worry less...the time my eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin...

...other great tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One...describe some of the many ways you can approach finding someone to give you advice and guidance. Second... the more potential mentors you will identify. Finding potential mentors is one of the many reasons to...group mentoring program in many of its chapters. Find out what other groups have going on locally. Your...good coach can make a big difference, if you can find one. Create your own peer mentoring circle. This..., “This was really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships...

...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-... your model system? Turns out that with light-inducible photosensitizers and a quick zap of the proper...case). The fluorogen is not fluorescent until it binds its protein partner thus forming the full FAP-TAP...FAP-TAP complex where dL5** is the FAP. It’s job is to bind MG-2I, a photosensitizer fluorogenic dye.  MG-2I...MG-2I-dL5** He et al tested out light-induced cell ablation with MG-2I-dL5** in two different experimental...present 96 hours post treatment (see figure 2). Induced cell death was confirmed by TUNEL staining.  The...ablation efficiency, but dL5** phototoxocity is induced when expressed on the cell surface, in the cytosol...

...beautiful Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge...Have you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in...badge, you’ll know that within that article you’ll find new plasmids. Simply click on the badge to be taken...them in experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical...are browsing article abstracts you should see the “Find Plasmids” box on papers that have deposited plasmids... find plasmids...

...! The starting point: Ligation-independent cloning Ligation-independent cloning (LIC) was first developed... personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does...specially designed plasmids. Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received...His new method, named sequence- and ligation-independent cloning (SLIC), eliminated many of LIC’s constraints...PubMed. SLIC: a method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol....guide to SLIC Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing...