We narrowed to 697 results for: Tec

... and bioinformatic technologies are used.  Improvements to sequencing technologies and bioinformatic analysis...the sequencing technologies that Addgene uses in the QC process, when these technologies are used, and ...up with the constantly updating technologies. Sequencing technologies used at Addgene Sanger sequencing...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365...using an Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited ...Sanger, was the first widely used DNA sequencing technology. The process relies on “sequencing-by-synthesis... Figure 1: Overview of Sanger sequencing technology. Created with BioRender.com. Sanger sequencing...

...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...brainstorming new technological innovations. Let’s take a look at three historical techniques: southern blotting...classic techniques that allowed for many seminal scientific discoveries (5, 8). While newer techniques have...these, these techniques still have useful applications and have informed many current techniques. For example...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress... time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing classic...inventor Edward M. Southern (1), can be used to detect DNA identity, size, and abundance. If a scientist...

...research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized...Luciferase-based tools are fantastic examples of technologies that can accelerate your research. The creation... Oplophorus luciferase by Promega’s Advanced Technologies group as well as evolution of the coelenterzine...RLuc reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR...I’ll cover two great applications of the NLuc technology. The first couples NLuc to fluorescent proteins...require external light sources. Unfortunately, detecting traditional luciferases’ dim light emissions requires...autofluorescence, and perturb biological phenomena. The technique of coupling FPs with Luciferases can overcome ...

...to two days.  Indirect or direct detection method Indirect detection is the most common way to run a western...choices to make when designing the experiment. What detection method? What membrane? What should you block with...for your experiment. But if you understand the technical aspects of a western blot, you'll be able to understand... so let's dive in!      Figure 1: The technical decisions to make when designing a western blot... western blot. It allows you to detect relatively low levels of antigen, while providing flexibility in... the more common chemiluminescent methods for detection (though the enzymes used for chemiluminescence...specific protein(s) of interest.  Determining linear detection range If you are quantifying your western blot...

...companies have real scientists answering their technical support questions (besides Addgene, I know both...emails and phones). We can’t be experts on every technique or experiment, but a picture of a gel or a sequencing... is answerable, particularly when it comes to technical questions. BUT, I do believe that scientists who... technical support...

...dramatic spread of the Cas9 technology: "The rapid adoption of the Cas9 technology was also greatly accelerated...you think these technologies are going? What is the next hot molecular biology technology? Have you deposited...unique position to observe and quantify how new technologies are being disseminated through the scientific... plasmid sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander...

...get all the attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin... states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid,..., likely due to limitations of current means to detect RNA-protein interactions. To address this need,... Stanford created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin...but it’s toxic to tissues and model organisms. Detecting RNA-protein interactions with RaPID RaPID is similar...*s in the context of RaPID for their ability to detect a known RNA-protein interaction.  In as little ...a scrambled RNA motif control). BioID2 had no detectable signal at the same time point. E. coli’s BirA...

...ability to create a DNA detection platform called DETECTR. How does DETECTR work? DETECTR works similarly to...samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for simple...published this in Nature Biotechnology. Like the SHERLOCK detection system, DETECTR has not been approved...nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements...nucleic acid detection however, either lack the sensitivity or the specificity to detect nucleic acids...CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both SHERLOCK and DETECTR harness the promiscuous...atomolar levels, within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity...

...names for technical support roles. Examples are Technical Service Representative, Technical Support Specialist...these departments from technical support. Technical support careers in brief Technical support careers are...worked as a technical support specialist for 4 years. Read on for insights from my time in technical support...products successfully. In technical support you may have to answer technical enquiries such as: “Can I... areas of expertise in technical support positions. When applying for technical support positions, you...familiarize yourself with technical support. Even having personal experience with technical support through the... do well in a technical support role. What general characteristics do jobs in technical support have? ...

... sectioning techniques due to a higher photon flux via the more efficient camera detector and lack of ...been a very slow imaging technique but recent advances in scanner technology and parallelization (7) have...sports photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the ...emerged as the dominant light microscopy contrast technique in modern biology (1). When performed correctly...block out of focus fluorescence from reaching a detector or restricting fluorescence to specific excitation...super-resolution (3). Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent...Microscopy was the first fluorescence microscopy technique to incorporate optical sectioning. Optical sectioning...

...alteration. Molecular cloning techniques are some of the most widely used techniques in the biological sciences...restriction cloning is one of the oldest of these techniques and is a multi-step process consisting of digestion...performed by many labs, a variety of other cloning techniques with higher efficiency and simpler procedures... still encounter several struggles with these techniques: unwanted mutations due to excessive PCR cycles...FastCloning is a simpler yet reliable cloning technique that was developed by Li, et al., in 2011. This... inserts. However, with any PCR-based cloning technique there is a chance of introducing an unwanted mutation...some tips for high efficiency cloning with this technique: Amplification efficiency is highest when using...

...toolkit based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a...continually impressed with the amazing plasmid technologies developed by our community of depositors. With...domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split" cas9...plasmids 62887 & 62888) Zetsche et al., Nat Biotechnol. 2015 Feb 2;33(2):139-42. doi: 10.1038/nbt.3149... system. The ease and flexibility of the LACE technology makes this system widely accessible for many ...imaging and inducible gene expression Brilliant technologies adopt basic biological concepts and employ them... the Weissman lab have teamed up and advanced technology in the fields of single molecule imaging and ...

...Addgene which allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These... Looking for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et...Jan 3;343(6166):84-7. Koike-Yusa et al., Nat Biotech. 2013. Dec 23.   Highly bright and extremely...new tool for peptide-peptide locking. The new technology is known as SpyLigase and is a protein domain...E690-7. For further reading about SpyLigase technology, read the Addgene interview with Mark Howarth...events occur in a physiological setting. Genetic techniques typically rely on overexpression or knock-down... and his team at the Institute of Science and Technology Austria, decided to take a novel approach to ...

...network with other technicians/company representatives. I got to talk with a technician from Neon Therapeutics...looking to try out a new technique or reagent! Racheal Komuhendo is a Lab Technician at Addgene who is interested...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ... we get many opportunities to learn from other biotech companies at workshops and can use what we learn...lab and office space shared by numerous emerging biotech companies. The space was modern with clean bold...heard about how LabCentral aims to enable emerging biotech companies, I was excited to go there because both... NEB gave a brief presentation describing the technology. Then we split into pairs to actually try out...

...place to provide community protection. Toehold switches for pathogen detection In 2014, as postdoctoral...these combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic...also demonstrated our technology on active virus, as well as shown that we can detect the virus in a clinically...Harvard University), we developed two new enabling technologies for the field of synthetic biology. The first...for the rational design of RNA sensors that can detect virtually any RNA sequence (Green et al, 2014)....sensors to be used outside of the laboratory. The detection threshold for these RNA sequences (3 nM), however...blood, urine). Adapting toehold switches for Zika detection We and other members of the lab had been working...

...engineering through biotech. After graduating with a masters of science in Horticulutre Biotechnology, he did his...his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of...works as a lecturer in molecular genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe...sciences and agriculture 5:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10...Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's current...

... In Situ Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of...Scientists routinely use techniques to alter gene expression or to label specific cells, but there are...experience is focused on classical embryology techniques such as basic observation and dissections. Students...Students don’t usually perform more modern techniques used in genetics or molecular biology because the...through time or after genetic manipulation. The technique involves using synthetically made RNA (riboprobes...binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added to the tissue...labs to bypass these steps and focus on the WISH technique. The expression patterns of 11 different planarian...

...proteins to study. These are highly useful for the biotech industry, which is in the process of capitalizing...other modifications to their bases in order to protect their DNA from host cell nucleases (4). To further...event that genome sequencing can be done, this technique can also help pinpoint the genes within a sequenced...live cells The following is a description of a technique published by Simpson, Sacher, and Szymanski in...membranes well with PBST to remove unbound cells. To detect bound cells, simply lay each membrane on an agar...

... distributed plasmid technologies pSV-PGC1 - Ok, it's not actually a technology, but pSV-PGC1 was Addgene's...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...give you an inside look at the Top 10 plasmid technologies distributed through Addgene over our first 10...genetically encoded calcium indicators were the hot technology of the year in our collection. CRISPR/Cas9 - ...Year of the CRISPR." The newest genome editing technology has been used and modified by so many labs and...

....   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity...R​eporter un​LOCK​ing) technique Sensitivity: Single molecule per milliliter can be detected – in the zeptomolar...-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as low as 1.2 copies...visualization. In this technique, however, instead of using lateral flow strips to detect the cleavage of fluorescently...: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses FnCas9, a protein that...CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587...times, have led researchers to turn to other technologies like CRISPR. Figure 1: An overview of...

... complementary strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes its annealing...bacteriophage, which is not a common molecular biology technique. However, an advantage of this method is that ...

...approach you choose, it’s essential to consider the technical limitations of each approach and to follow best...

...do this.” Biotech startups are only successful if they are 100X better than current technology and get ...Capital in Biotech  - Posted on Forbes.com, contributed by Bruce Booth How to Find a Job in Biotech and Resume...developed during graduate school and turn it into a biotech company. Transitioning an idea from academia to...Sample6 is an enrichment-free bacterial pathogen detection system that uses synthetic biology to target and...concepts to engineer bacteriophages and use those to detect unwanted bacteria. How did graduate school help...a succesful entrepreneur? As a co-founder of a biotech startup, Mike explained, his career transitioned... the best product-market fit for this set of technologies, effectively communicating the advantages embodied...

...the limited technologies available. Over the past few years, however, remarkable technological advances ...revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists...mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics” was coined...fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting fusion gene... functional topography of the neural circuit architecture of the brain regions associated with motivated... able to distribute materials containing FLEx technology. Read the genOway press release for more information...Deisseroth, Karl, et al. "Next-generation optical technologies for illuminating genetically targeted brain ...

...Branon et al. Nat Biotechnol. 2018 PubMed PMID: 30125270. Changing the way we detect viral outbreaks ...often escape detection. The Alexander Green lab found a solution: a low-cost, cell-free detection assay that...isothermally, and detected through a toehold switch, an RNA sensor that can be used to detect the presence ...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be... have now applied the same directed evolution technique to the biotin ligase from which BioID was derived... and BioID. In the Ting Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, ...contributed by Izzy Mueller  Listen to the viral detection podcast segment As the primary cause for both...

...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that occur...primary methods of off-target detection: biased and unbiased. Biased techniques only sequence certain sites...: Biased Genotyping Options Technique Sequence Information Detection Limit Advantages Disadvantages...highly sensitive with detection levels as low as .01% (Hendel et al. 2015). Low detection rates mean the investigator...off-target mutations if they remain undetected using these techniques. Frequencies of off-target mutations...have a minimum detection limit of 0.1% (Tsai et al. 2015). This contrasts with detection frequencies of... Table 2: Unbiased Genotyping Options Technique Detection Limit Application Advantages Disadvantages...

... for detection RANbody is detected enzymatically with HRP substrate More sensitive than detecting antigen...require a secondary antibody for detection. Instead they are detected with tyramide signal amplification...and is useful for detecting GFP. Spaghetti Monster tagged RANbodies can be detected either with a labeled...sensitive than HRP RANbody detection, it is approximately as sensitive as detecting the same antigen with ...RANbodies are easily detected with a labeled anti-chicken antibody. Chicken IgY RANbody detection is less sensitive...sensitive than HRP RANbody detection but approximately as sensitive as antigen detection with a standard primary... When detected with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen...

...different biotech companies which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma...irreplaceable or highly expensive). Detecting mycoplasma contamination Detection of mycoplasma by naked eyes ...Another method used for mycoplasma detection is using PCR. In this technique, PCR primers specific to the 16s...cells that can detect the presence of mycoplasmas in the culture. Once these cells detect mycoplasmas, ...Assistant at MGMs Institute of Biosciences and Technology. If you are reading this article then you probably...through cross-contamination and due to poor lab techniques. These include reusing pipettes for multiple ...turbid nature, contamination is difficult to be detected in the serum that is most commonly used for every...

...that require scientists to detect proteins in their samples. One technique that relies heavily on antibodies...Enzyme-linked Immunosorbent Assay. ELISA are used to detect proteins within a 96- or 384-well, flat-bottomed...bottom of the well), and then its concentration is detected through visualization of an enzymatic reaction...ELISAs, which differ in the way the antigen is detected. These are sandwich, competitive, direct, and ...available ELISA kits are for a sandwich ELISA. This technique works by first coating the wells of a flat bottomed...specific to the protein you are interested in detecting in your sample. When an ELISA kit is ordered, ... your protein of interest.  The next step is to detect the protein-antibody pair. In a sandwich ELISA,...

...Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information Tech Transfer News: A Reintroduction...get an Addgene account for Tech Transfer Offices. They’re packed with tech transfer resources (summaries... question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...stats and strategies with tech transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein...’re trying to make MTA turnaround faster with technology transfer offices (TTOs). The latest of these ...deliver the very first ones in person by our own Tech Transfer Team! Why MTAs Matter Though they may seem... important—they guarantee certain rights and protections for depositor and requestor alike. That’s why...

...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

...cannot imagine any use for GM technology other than to sustain those techniques. We feel strongly that the...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...

...genetically encoded photocaging technique to insert a light-removable protecting group, specifically a nitrobenzyl...have been making major improvements to CRISPR technology since its initial applications for genome engineering...prime editing, anti-CRISPR proteins, and other techniques all aim to improve targeting specificity or reduce...2015, scientists combined CRISPR and optogenetic techniques to develop a variety of photoactivatable CRISPR...spatially control gene expression. Both of these techniques take advantage of light-induced dimerization ... their CRISPR system. This common optogenetic technique can be employed in other split systems where light...activated with light and lowered background DSBs (non detectable). Like their prior work, the Sato lab also showed...

...LDR can reliably detect single-base differences. The original creators of this technique visualized the ...Development of a ligase detection reaction/CGE method using a LIF dual‐channel detection system for direct ...-based ligase detection reaction assay using a molecular beacon probe for the detection of low-abundance...other thermostable DNA ligases. These applications detect specific nucleotide sequences with high specificity... (SNPs) (5,12,13). For example, in the Ligase Detection Reaction (LDR), probes complementary to the sequence...methods to greatly increase the sensitivity of detection (14-20). LDR has also been extended to multiplexed...multiple potential SNP sites (15). The related LCR technique uses two sets of probes to achieve exponential...

...labeling strategy to detect cell-cell interactions in vivo (Ge et al., 2019). This technique relies on an S.... with biotin using avidin coated purification techniques. Due to the high affinity of biotin for avidin...proteins The first biotin based proximity labelling technique, BioID, was developed in 2012 in the Brian Burke... developed as well as new proximity labelling techniques. These newer tools are also currently available...combines a protein fragment complementation (PCA) technique with proximity labelling to validate binary protein-protein...proteins in one simple experiment. PCA is an in vivo technique where two proteins are fused to complementary ...for Split-BioID RaPID: RNA-protein interaction detection So far, we’ve covered ways to study protein-protein...

...emerging technology. We could do this for CRISPR/Cas9 – or any other germline editing technique—too. As... of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist... is unethical to use scientifically unproven technologies to edit a human embryo, or have a woman carry...published on December 13 shows us, CRISPR/Cas9 techniques are continually being refined; it is completely...will eventually have available genome editing techniques that are sufficiently safe to manipulate an embryo—even... the June 2012 report on the ethics of “Novel techniques for the prevention of mitochondrial DNA disorders...disorders.” This document is about the popular technique incorrectly known as “three-parent babies,” where...

...mixture of cells. The technique of using fluorescently-labeled antibodies to detect proteins is referred...whole organs. Because the tissue architecture is preserved, this technique allows you to not only visualize...the same experiment because the reagents used to detect the presence of an antibody depends partially on...the primary antibody and provides the method of detection. You’ll also want to use the appropriate controls...applications. Western blot Western blots are used to detect the presence of proteins from samples containing... a secondary antibody allows the protein to be detected by chemiluminescence or fluorescence. Western ...immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single protein from a heterogeneous...

...system, detect post-translational modifications, quantify protein levels relative to standards, detect the..., or western, is one of the simplest methods to detect the presence or absence of a protein (Renart et... size, (2) transferred to a membrane, and  (3) detected using antibodies.   Figure 1: Overview...finally visualized.   This process allows you to detect a single, specific protein within the complex mix...cells or tissues. Westerns are useful not only to detect the presence or absence of a protein, but can also...validated for immunoblotting. Different immunoassays detect proteins in different states. For example, in immunoprecipitation...protein in its native state while a western blot detects denatured polypeptide chains. The protein regions...

...enzymatic signal detection, users provide a substrate that the enzyme converts into a detectable product. Scientists... in a sample and then a second detection antibody binds. The detection antibody can be directly conjugated...then adds an antigen-specific detection antibody.  Importantly, the detection antibody and the capture antibody... either directly conjugated detection antibodies or unconjugated detection antibodies followed by conjugated... specificity, and sensitivity (a good ELISA can detect picogram quantities of the target!) and use them...conjugated with a reporter or tag that can then be detected.  Typically, the reporter is an enzyme such as...abundance antigens may want to consider alternative detection methods.      Figure 1: The four types...

...enrichment and structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered...these limitations, the Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC...and in complex with its chaperone NPM2, but the technique should be widely applicable to other targets. ...doi.org/10.1101/2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge freezing...proteins that naturally adsorb to the AWI, can protect cryo-EM samples from damage (Abe & Lim, 2024).  ...complexes may dissociate  (right). B) Protein samples protected from AWI by helical LEAs. C-E) cryo-EM single-... Lim, 2024 under CC-BY-NC-ND 4.0 license. Protection by the LEAs allowed the authors to determine ...