We narrowed to 645 results for: Rest

...When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone... of how to do perform restriction digests.   Overview of the restriction cloning process. Both ...) and the DNA sequence of interest (green, insert) are cut with restriction enzymes to generate compatible...backbone will contain a variety of restriction enzyme cut sites (restriction sites) downstream of the promoter...DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently...backbone covalent.  Before beginning the restriction digest and ligation process, you should carefully...these both must have compatible cut sites for restriction enzymes that allow your insert to be placed into...

...methylation may affect your restriction digests. Why methylate? Restriction enoducleases are so ubiquitous...It turns out that restriction enzymes are one half of naturally occurring restriction modification systems... from the restriction modification systems, yet can still affect whether certain restriction enzymes can...associated with any restriction modification systems, their sequences may overlap with restriction sites, inhibiting...Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected results...sequences to prevent them from being degraded by restriction endonucleases. A given prokaryote typically has... has genes encoding one or a few restriction modification systems containing methyltransferases that add...

...allows for the isolation of a genomic region of interest targeted by a gRNA. CRISPR-mediated purification...3xFLAG-dCas9) and a gRNA targeting their genomic locus of interest, the promoter region of the transcription factor... region alleviates these issues as a region of interest can be targeted using a gRNA, which can easily...system can be used to isolate genomic regions of interest with dCas9 and a targeting gRNA. One such example...

...transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead of settling...terms of both types and topics of work. A younger interest in being a writer kept chirping up. The evolutionary...In my role as an editor at Science, my various interests come together as useful and productive, forming...forming a different constellation of interests and skills than what fits for applied research. I focus in ...Science communication opportunities If you are interested in the science communications world, you might...more planets to choose from. Put your skills and interests into your backpack, and step out there into the...

...plasmids. Here, we'll cover restriction digest analysis. Diagnostic restriction digest Diagnostic digests...Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with restriction enzymes...quick overview of how to analyze a restriction digest:     Restriction digest tips and tricks: The following...Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into your functional...organization of different features within the plasmid. Restriction analysis can also be used successfully even if...relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm the presence...backbone. To do this, you'll use enzymes with restriction sites that flank the insert. You will need to...

...you’re on a break from expressing some genes of interest).   Blugene is so excited they can’t stop...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...particular cell types. For example, the mDlx enhancer restricts expression to GABAergic interneurons. These vectors...

...peak fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, while...responding variant. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging small ...

...access to the antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as...

...performer if they are enough of a jerk to make the rest of the team miserable.   A good manager keeps...dissatisfaction, the work can get done, and get done well. The rest of the blogs in this series will address more specific...

...out our referral form and we’ll take care of the rest. Know of a useful plasmid that’s not in the collection...

... China was affected by COVID-19 earlier than the rest of the world, from mid-January to mid-March.  To... of all of our plasmid orders, we will focus the rest of our discussion on these three regions. Each of...indefinitely due to COVID-19. In our lab, we were interested in examining the effect of COVID-19 on worldwide...graduate student at Harvard University. He is interested in using selection, evolution, and machine learning...

...when ready, and we’ll take care of the rest.    We think Packaged on Request AAVs are a big deal, so if...request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request, you...processes involved; simply request your plasmid of interest in your desired serotype, place your order when...

...issued by Google in October entitled “Rise of the Rest: The Growing Impact of Non-Elite Journals” found...for a moment, it’s clear that articles in less-prestigious journals have been gaining ground. A study issued...proportion of highly cited articles appearing in lower-prestige journals has risen steadily from 1995 to 2013.... in citations for older publications. (As an interesting aside, Nature recently reported that the most...

...compared to untagged GFP overexpression, while the rest of the fusions had some negative effect on these...help you determine localization of a protein of interest, purify it, or determine its expression level ... with CRISPR Fluorescently Tag Your Protein of Interest ...

...passion. Do what you love. Don't worry about the rest.”- Jason Snair, Product Designer On the job search...most of the most interesting jobs are never advertised. You have to meet interesting people and maintain...

... a better understanding of the phenomenon soon.  Rest assured, as the PUF protein design tools continue...devise RNA binding proteins to hit any target of interest. The new tool was developed and implemented by...specificity engineering. “The RNA binding domain is interesting because by changing certain amino acids you can...structure of RNA. As a result, targeting the RNA of interest requires that the RNA be tagged. “This tool makes...the PUF Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves...

...your materials under the UBMTA terms, so you can rest easy knowing your rights – and the rights of your...

...Mr. Incredible, an added bonus. You can find the rest of our September posts in chronological order here... phage proteins that bind to your bacterium of interest. For those of you who are not microbiologists,...

... exception - the rest of the lab team helped to edit and add to this post. Interested in learning more...

... cells that contain a specific gene survive. The rest do not grow. While there are many ways to design...You’ve worked hard to purify your gene of interest, get it into your plasmid backbone, and zap the mixture...some now have plasmids that contain your gene of interest, but others will uptake plasmid backbones that...antibiotic resistance cassette along with your gene of interest. In these cases, antibiotic selection will select... if the plasmid backbone contains your gene of interest. Auxotrophy Besides antibiotic selection, you ... select for a successful uptake of your DNA of interest by taking advantage of bacterial characteristics...by recombination sites. To introduce a gene of interest into the destination plasmid, recombination sites...

...solubility tag GB1. In SunTag nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum...fusing a fluorescent protein to your protein of interest, you instead fuse it to the synthetic SunTag scaffold...

...comprehensive pre-compiled downloads and via a full-featured REST Web Service API. Both options are fully open access...CRISPR results for any gene of interest. In addition, users interested in a particular disease, cell type...literature for a drug, disease, or condition of interest using a simple keyword search. For example, searching...entire CRISPR database for particular screens of interest.   Figure 3: Each publication featured ...access and available without any licensing restrictions.    As CRISPR technology continues to develop and...

...and we’d love to know what you think. As with the rest of our services, you can bet that we’ll be continually...

...system. The effector is placed downstream of the rest of the SPARC components and expression is controlled...used to specifically manipulate a cell type of interest in an organism, all cells of that type are affected...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a...

...CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed by three of the biggest... weren't quite sure what to expect in terms of interest, but visitors to the poster were impressed to ...edge science in increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing...

...from the rest of our panel. Lastly, we want to detect the expression of our protein of interest: CD45. ...It also means we should exclude APC-Cy7 from the rest of our panel. Besides some background cell death...through genetic modification. As your protein of interest will only occur in combination with that reporter...antibody-fluorophore combinations for different markers of interest, you can separately detect these markers in your...

... with the virus. Then the viral particles do the rest! Downstream expression or integration resulting ...constructed viral vector containing the cargo of interest is then typically transfected along with viral...

...just go to sessions that you find interesting and not go to the rest.” Having an on-site conference canceled...started yet! @neurosymp will be interesting this year. Everyone interested in #Neuroscience should come ...aspect and I could plan more with regard to my interests.” Use the chat box and upvote If the thought of...

... as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique or the Flowtime Technique...

... now you don’t have to worry about operating the rest of the car as well. Similarly, cell free expression...keeping. Finally, if you are a fellow researcher interested in adapting your research ideas into hands-on...student at MIT in Biological Engineering and is interested in developing synthetic biology based tools for...

...FPbase data in their own projects and applications, a REST API allows programmatic retrieval of the majority...that serve to further characterize a protein of interest (and we very much appreciate even those small ...

...This variant exhibits high contrast between its resting state and its calcium-bound state, which was achieved...

...of curated plasmids from all over the world. The rest of this piece will describe plexWell™ in more detail...

...the PCR product Isolate your PCR product from the rest of the PCR reaction using a kit, such as the QIAquick...assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for...plasmid of interest. You can use similar processes to add overhangs to your insert of interest for Gibson...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...discussed in our restriction cloning post. However, even more than with standard restriction cloning, it is...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...itself are very similar to those outlined in our restriction cloning post with a few quirks specific to the...

...Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists Series Learn How... careful not to go too far. Is the assignee interested in this task? Don’t give out tasks – where possible...

...and screened by NGS to spot check the samples, the rest will be put into a status we call “Archived.” This...

... 180 regions to another or from the brain to the rest of the body. Therefore, by simply looking at how...

...the meeting and then communicate outcomes to the rest of their team. Here's a template for project meetings...

...and techniques. If you are interested in reading more, you can find the rest of the Plasmids 101 posts ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

.... The two remaining plasmids in the kit have the rest of the protein in place including FokI endonuclease...new gene variants associated with conditions of interest, then studying those variants in model systems...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...

...their precise location. The end of the heteroduplex rests against the RuvC nuclease domain, which stabilizes...the target DNA strand, makes few contacts with the rest of Cas9 and is highly flexible; it can rotate about...

...of R, is capped, and proceeds through U5 and the rest of the provirus. 3’ LTR Terminates transcription...plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by long terminal...

... translate that valuable information back to the rest of my team in Oxford (UK) to help solve those problems...realized it was a great fit for my skillset and interests. As such, I want to be able to share more about...the North America region. She is particularly interested in DNA sequencing tech, and the intersection ...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...

...Find lentiCRISPR v2.(If you’ve peeked through the rest of the list at this point, you may have noticed ...Zhang lab is used to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top ...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

.... We know; we asked each group to share with the rest of the workshop.  For Figure 1, Addgene chose this...

...target protein remains bound to the column while the rest of the proteins are eluted. Then, the antibodies...isolate antibodies only recognizing your protein of interest: affinity-purified antibodies are isolated from...

...not happen in a vacuum that is isolated from the rest of society or devoid of social context. This “general...process. My role at pgEd I have always been very interested in the societal aspects of science, even while...science communication or policy requires broad interest in issues at the interface of science and society...narrowly-defined research questions in our field of interest. We often communicate in very technical and jargon-filled...jobs and those of their families. Having a broad interest and awareness of issues outside of your field ...them. Researchers currently in academia who are interested in such careers should take advantage of their...Genetics Education Project (pgEd). He is broadly interested in the legal, ethical and policy issues at the...

...preparation should be doing your best to be well-rested and alert the day of the procedure. Mice move much...