...-2, but with many academic research labs closed during the pandemic, it’s hard to get these reagents. ...available through a repository, they remain accessible during these times. Centralized reagent repositories, ... rapid access to validated reagents, especially during a time when these materials may not be available...community will continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for ... Case study 3: Mouse strains Reagents developed during the SARS-CoV pandemic more than a decade ago are...Biotechnology 2020). The continued success of repositories, during the pandemic and in the future, depends on support...

...use more mentors to listen, guide and support us during training and throughout our careers. An easy way...

...like to go to. During the conference Take notes during talks. If you have questions during the talk, jot...conference depends on what you put into it before, during, and after the meeting. Let’s break it down into...Try to avoid jargon in this initial introduction. During the conference When you meet someone new at a conference...poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session early...the board and meet your poster session neighbors. During the session, if someone comes up to my poster, ...discussing with your lab. If you made a connection during the poster session, send them a follow up email...to turn to an established social media platform. During the conference If the conference has a social media...

...worry about traveling during an already stressful time, and I was more relaxed during both sets of interviews...yourself to a few members of your desired department during the application process. Many institutions recommend...in case one isn’t able to respond to your email. During my PhD application cycle, I reached out to around...faculty that you might be working with for the duration of your program and ask any questions that will... and make sure you cover everything you need to during the interview. (Virtual) Campus Visits After receiving...Through the virtual systems created and promoted during COVID-19, online meetings with current students...worked with students to maintain these time goals during COVID-19? How are students funded? Are there any...

...role was incredibly valuable because it was a set duration of only three months and made a good job to list...list on my CV. Three months was a perfect duration for me because it was sufficient to figure out if my...problem solving. I believe that is something lacking during degree courses; they never really prepare you for...failure and problem solving scientists experience during research. Working abroad in rural Africa Once this...another area which I was really passionate about during my degree. I loved parasitology. However, when ... held me back; I do not have a second language. During the recession there were many qualified scientists... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...

...data for lab procedures. With this data in hand, we can go back and optimize our procedure to make the ... biology is messy. Every single step in a lab procedure can and will fail. Some step failures require ...monumentally difficult without software. Tracking lab procedures also gives you access to hard data about what...definitely be interested to see which part of her procedures are the most failure prone. In an academic lab... Some steps fail “hard”, they reset the whole procedure. Other steps fail “soft” they simply need to be...might leave you sad and confused when a tricky procedure failed and one of your techs started over with... but the software should be mirroring the lab procedures, not artificially constraining them). In the ...

... if we were to restart the procedure we would not have enough time. During the following two weeks, we...summer interns presenting their capstone projects during the Summer Intern Poster Presentation.  As ...the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine...always came with surprises. Originally we thought during the first three weeks that we had made a mistake...not having the right concentration of a reagent during our HiFi assembly but decided to go ahead anyway...on my capstone project report and presentation, during the final week at Addgene. This report shared my...

...As Addgene believes many of the topics discussed during the symposium will prove incredibly important to... that the main question they wanted to focus on during the symposium was “How many graduate students/post-docs...problems in academic research and potential solutions During the first couple of sessions of the symposium it...not easily achieved. Of note, it was highlighted during the conference that just increasing funding for...Editors I wish I had been more cognizant of this during my PhD. Submitting a publication doesn’t have to...symposium was on diversity in academic research. As during previous panels, speakers Moon Duchin, Rafael Luna...researchers and, in fact, one of the projects developed during the Saturday Hack Day Session focused on finding...

...specific subset of cells in a population in a procedure termed fluorescence activated cell sorting or ...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...factors including sample preparation, staining procedures, and controls. To prevent instrument clogs, ensure...control with cells that went through the staining procedure minus the antibody. An unstained control allows...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...fluorescence-minus one panel will help you set up gating during data analysis.  We hope that this blog has provided...

...packaged inside the vectors during production. These impurities cannot be removed during the purification step...recombinase-independent recombination during plasmid growth in bacteria or during viral production. This recombination...wrong plasmid? What if bacteria snuck into the prep during the production process? These questions are especially...Evidence for Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and...

...or need a little assistance at some point during the procedure. Be an active partner Everyone’s learning...you don’t do proper preparation and pay attention during training, then you won’t be very proficient afterwards...how to prepare and things to keep an eye out for during training. Preparation before bench work Are there... the in-person training component. Documenting during training It’s hard to recall a lot of details, ...trainer? Adopt the schedule of your bench mentor during training. Don’t expect them to work around your...

...techniques with higher efficiency and simpler procedures have been developed. Some of these include, TA...annealed and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and...isolated from methylation competent E. coli for this procedure to work properly. Directly transform chemically...plasmid to confirm no mutations were introduced during PCR or any subsequent steps. Using high fidelity...have a better understanding of the step by step procedure of FastCloning, here are some tips for high efficiency...

...is based in the lab. If you’re unsure what to do during this period, check out these tips and resources...staying away from others.  Meditate. Meditating during the day is a great way to take a mental break, ...interactions and #social support are even more necessary during these times of stress due to #COVIDー19. Receive...looking for some resources specific to self-care during the COVID-19 pandemic, check out Care For Your ... you no matter what you’re trying to accomplish during this period. ...

...2016 and then working four more years in academia. During my job hunt, I quickly noticed one of the biggest... of your work, such as improving a process or procedure, solving a problem, or making a key decision. ...You’re going to be interviewed by numerous people during your industry interview. Each interview is an opportunity...you have their emails. You can use notes you took during your interviews to help craft these thank you's...remote interview. Fielding questions can be hard during this talk since you’ll be sharing your screen and...

...detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun Choe’s lab identified...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...repository for many years while many were developed during the COVID-19 pandemic. Our mission to accelerate....434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The CRISPR...Resources page Stay up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2 pseudovirus plasmids...

... cloning procedures. Use high fidelity DNA polymerase This will minimize random errors during PCR: we ...bacterial recombination pathway to allow any cloning procedure to be performed using a simple two-step, 2 hr ...be immediately adopted by any laboratory. All procedures are performed using the same three step protocol... greater homology, so performing more complex procedures can be aided by extending homologous sequences...

...will be able to enter your PO number as payment during checkout. Placing your order online has some benefits...Add all your items to your cart. Click checkout. During the payment step of checkout, you will have the... invoice to the billing address that you listed during checkout. This invoice will reference your Addgene...Addgene order number and PO number that you entered during checkout. At this time, your organization will ...

...large scale cell mobility are essential processes during embryonic development. These are required for a...)? How do blood monocytes patrol blood vessels during immune responses (Auffray et al. 2007, Finsterbusch...Dynamics of neutrophil migration in lymph nodes during infection." Immunity 29.3 (2008): 487-496. PubMed...Jackson G., et al. "Macrophage and T cell dynamics during the development and disintegration of mycobacterial...Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog ...