Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 237 results for: FAS

Showing: 1 - 20 of 237 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... Restriction Cloning and Fast Cloning Requirement Restriction Cloning Fast Cloning Choose Restriction... Transformation + + The FastCloning process in brief In FastCloning, you PCR both the vector and ...and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and vector...most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning technique that...understanding of the step by step procedure of FastCloning, here are some tips for high efficiency cloning...of the colonies are correct. Good luck with this fast and easy technique! Many thanks to our guest blogger...PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    .... https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...doi.org/10.1038/s41586-019-0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani.... https://doi.org/10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. ...Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers...25692716. PubMed Central PMCID: PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...such as 2-fluoroadenine (2-FA), organisms with functional APT will convert 2-FA to cytotoxic nucleotides...nucleotides, resulting in death. In the presence of 2-FA, potent gene silencing of APT is required for survival...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-FA (2020) Imposter syndrome threatens diversity. Science.... https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 ....

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino...breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principal means...

  14. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...Like everyone else in the world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember...

  17. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...The idea was to evolve a TadA-7.10 version with a faster deamination rate so that deamination can happen...PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems allow for rapid continuous...speed dictates which phage variants can propagate faster than the rate of dilution (passage from the infected...Created with BioRender.com. Introducing ABE8e - a fast and flexible adenine base editor Lower-stringency..., they identified TadA-8e, which edits 590-fold faster than TadA-7.10. It is compatible with all Cas9 ...resulting base editor was named ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct...functional domains speed up the development towards faster and more precise base editors capable of editing...

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement: NGG and an expanding number...has anti-viral application potential as a result. Fast facts – PAM requirement: AAG or TTC  Best for: very...optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or TTTN. Best for:...variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best for: high fidelity...information on Cas13, check out our blog on RNA editing. Fast facts – PAM requirement: protospacer flanking sequence...11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer flanking sequence...targeting single stranded RNA.   Table 1: Fast facts on Cas family proteins and genome editing ...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... below use the following optogenetic modules: VVDfast homodimerizes under blue light iLID-SspB heterodimerizes...limitations (Figure 1). Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of...of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and SspB-mCherry-p60 (katanin) are... of two separate blue light-sensitive systems, VVDfast to control recruitment of an “anchor” to microtubules...anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module and the SspB-mCherry-p60 katanin...controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and other optogenetic modules...

  20. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Verkhusha Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow...superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein...mScarlet Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins...requires some time for chromophore maturation, splitFAST displays rapid and reversible complementation....
Showing: 1 - 20 of 237 results