We narrowed to 270 results for: FAS
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TypeCollection...Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem...Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino...Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F....genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...
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p53 Pathway
TypeCollection...superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post... Restriction Cloning and Fast Cloning Requirement Restriction Cloning Fast Cloning Choose Restriction... Transformation + + The FastCloning process in brief In FastCloning, you PCR both the vector and ...and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and vector...most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning technique that...understanding of the step by step procedure of FastCloning, here are some tips for high efficiency cloning...of the colonies are correct. Good luck with this fast and easy technique! Many thanks to our guest blogger...PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...effects can mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using ...constructed as well. They called this library the CTD2 FASMIC Plasmid Collection for “Functional Annotation of... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...high levels of correct assemblies) translate to faster assembly workflows that require less screening ... -
CRISPR History and Development for Genome Engineering
TypeCollection...Nature . 550(7676):407-410. PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...CRISPR-Cas9. Cell . 167(7):1829-1838. PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post.... https://doi.org/10.1038/nmeth.3993 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...doi.org/10.1038/s41586-019-0908-x Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani.... https://doi.org/10.1016/j.cell.2014.02.001 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. ...Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers...25692716. PubMed Central PMCID: PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...such as 2-fluoroadenine (2-FA), organisms with functional APT will convert 2-FA to cytotoxic nucleotides...nucleotides, resulting in death. In the presence of 2-FA, potent gene silencing of APT is required for survival...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize... -
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A Guide to Getting Started in Undergrad Research
TypeBlog Post...doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-FA (2020) Imposter syndrome threatens diversity. Science.... https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 .... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post.... References and resources References Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet... containing the recombined product tend to grow faster, so we recommend picking the smaller colonies which... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...AR. Nature. 2013 Jan; 493, 429–432. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F.... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino...breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principal means... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...556:57–63 . https://doi.org/10.1038/nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...that better human health. References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...Like everyone else in the world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...https://doi.org/10.1016/s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F ...