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We narrowed to 34 results for: PRS

Showing: 1 - 20 of 34 results

  1. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available...these planarian species. These plasmids all have a PR-T4 vector backbone, which is a modified version of...sequences for creating riboprobes and dsRNAs into PR-T4P. The Sánchez lab protocol also provides step-...

  2. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... the open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more ...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...Repressor cI inhibits transcription from phage promoter pR, preventing expression of a reporter gene. When light...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...https://doi.org/10.1038/s41541-022-00503-y Gorry PR, McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont...

  10. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid...plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. ...

  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn Off Genome Editing Single-chain Cas9 photoswitches... saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly... study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques each employed a photoactive...

  12. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...already. I know we've discussed it before, but CRISPRs allow research to move so fast." On the very same...microbiome alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists...

  13. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...and more.  “In the past, genome engineering with CRISPRs was limited to modification of a few loci at a ...Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to Edit Disease Out of the Genome...

  15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Sontheimer and Davidson labs discovered three anti-CRISPRs for a type II system in Neisseria meningitidis ... here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins are highly diverse but most block CRISPR...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus! From the lab of Yonglong Chen, pCS2...genome editing system that combines TALENs and CRISPRS. Developed by David Liu and colleagues, FokI-dCas9...

  18. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...CRISPR including: Base editing Prime editing Anti-CRISPRs … you name it! So get excited and download the...

  20. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad...
Showing: 1 - 20 of 34 results