...Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity for... 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral vectors...hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539  AAV5  pOTTC1596 - pAAV SYN1 HA-hM3D...

...Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496...serotypes. This collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465... to give scientists access to a wide array of promoters and fluorophores for validating and testing purposes...-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs strong, ubiquitous expression. This is...

...work with many cell types, as CAG is a ubiquitous and constitutive promoter. Use this for doxycycline inducible...pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-tTA AAV2...repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls...

...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter...Human Synapsin-1 promoter. Good for neuronal expression. hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression...Synapsin-1 promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for...Podgorski Lab has provided a brief breakdown of the promoter systems you can use to express the iGluSnFR3 variants...

...potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714...collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO...

...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has tested...imporant is that, upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI restriction sites...

...interest. Promoter The promoter drives the expression of the AAV transgene. On a plasmid map, promoters are ...location. Promoters can also drive different levels of expression. For example, CAG is a strong promoter that...synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer plasmids have a promoter, but...are upstream or 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can...nuclear export of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located...for the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells...

... trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   Primarily used for RNA     ...commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter Constitutive.... In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This ...post on viral vector parts. Promoter specificity Aside from choosing a promoter based on type of RNA transcript...cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...

...rat neurons over other promoters, including ubiquitous ones such as EF-1α and CAG. Figure 2: Dual...plasmids used to express CRE under neuron-specific promoters (aCaMKII and hSyn). Created with BioRender.com...plasmids used to express Cre under neuron-specific promoters, including calmodulin-dependent kinase II alpha...excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be found here. Neuron-optimized...expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability to induce CRISPRa...consequently regulates neuronal physiology by promoting the development of neurons and synapses as well...cells to determine which transcription factors promote neuronal differentiation (Liu et al., 2018). The...

..., the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and rabbit... tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable markers...

...Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 ...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...more accurate cell and tissue targeting. While promoters and AAV serotypes can sometimes be used to target...

...neuron-specific expression (hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version ...

...your experiment site or circuit can occur. Promoters The promoter controls how the gene of interest will ...selective, or inducible.  Ubiquitous promoters  These often-strong promoters offer the most flexibility for ...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...vitro) or months (in vivo).  Selective promoters Selective promoters, such as synapsin, glial fibrillary ...International License.   Inducible promoters  This type of promoter allows you to decide when to turn ...three key variables of an AAV vector: serotype, promoter, and titer.     Figure 1: Flow chart depicting...depicting a) steps for selecting serotypes and promoters to test in your AAV system and b) steps for testing...

...the injection site. Test the promoter It is also essential that the promoter be thoroughly tested in your...your cells.  Some promoters, such as CAG are very strong and may require less viral particles or relatively...cell-type specific” promoter, such as the truncated tyrosine hydroxylase (TH) promoter for catecholamine...open reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...what can we do about it?  Test the serotype and promoter The first step is to know how the AAV vector you...includes a thorough testing of both the serotype and promoter with in vivo experiments to determine the optimal...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low...

...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown. Vector provided ...

... AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...quite pronounced, even when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its tropism...transgene expression. Gene regulatory elements [promoters and enhancers, miRNA target sites, and recombination...over systemic delivery. Otherwise, cell-specific promoters and enhancers (Mich et al., 2021) can help restrict...optogenetic and chemogenetic tools. Dual expression Promoters, miRNAs, enhancers, and switches all take up precious...particular experiment is determined by the capsid and promoter/enhancer strengths in the cell type of interest...candidate regulatory elements such as enhancers and promoters for cell type-specific targeting (Chan et al.,...

...′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing...cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target...cloned directionally into the BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330...Once amplified, you can insert this region into a pCAG-EGXXFP plasmid using standard cloning techniques...of transfecting HEK293T cells with your modified pCAG-EGXXFP plasmid along with the individual gRNAs in...Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are the perfect primer sets...

...integration is TA dinucleotides and it leaves behind the CAG DNA footprint from its terminal sequences at the ...example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased to insert ...

...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al....known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...

... gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page... over 30 promoters and 140 genes, including constructs with unique tissue-specific promoters expressing...genomic libraries are cloned downstream of a minimal promoter sequence. Should one of these random stretches...activate its own transcription from the minimal promoter and its strength can be determined from its enrichment...variety of plasmids containing many different promoters, RBS’, coding sequences (CDS), and terminators...fluorophores, generate expression vectors with various promoters suitable for in vivo expression, and/or produce...mice. The Jacks Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus...