We narrowed to 25 results for: dox

...presence of Dox. In this case, your gene is repressed in steady state and is expressed when Dox is added ...absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, ...activates the expression of your gene. The addition of Dox in the culture will then repress your gene of interest...activity and it requires continuous administration of Dox to repress transgene expression. In contrast, in ...

...two PiggyBac integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette...contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression. When ...Next, perform a short (12-hour) induction with doxycycline prior to FACS, gating for the top quartile (or...producing cells. After FACS, return the cells to doxycycline-free media and expand the cells. Use a subset...engineered cells via regular intraperitoneal doxycycline injections to enable their tracking with ultrasound...

...from Abhi Aggarwal.   Brandán Pedre Pérez: Redox biosensors Brandán Pedre Pérez is a postdoc at ...Cancer Research Center (DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live...cysteines near the chromophore that are susceptible to redox modification, the MPST hydrosulfide can be transferred...improved biosensors or biosensors that detect new redox metabolites are vital for researchers to better ...

...genome editing system that could be regulated by doxycycline. Now, they’ve developed another AAV toolkit -...system is regulated not only temporally with doxycycline, but it can also be regulated spatially with ...

...expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful when you...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman Primates...

...control of the TRE promoter, which allows for doxycycline-inducible expression. The end result is genomic...controlled expression of the dCas9-VPR transgene by doxycycline in your target cells. This plasmid adds to the...

...ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also...

... prefers expression in E. coli, the use of a thioredoxin A (TRX)-containing vector can improve disulfide...

...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors...

...gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase is proving an...

... levels of XFPs - Reduce the amount of tTA. Paradoxically, too much tTA leads to a reduced expression ...

...Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired gene (whose...

... per generation, and (2) the addition of the thioredoxin-binding domain (TBD) from bacteriophage T7 DNA...

...target cells by the addition of tetracycline/doxycycline to the grown media resulting in target gene knockdown...

...factors, environmental conditions such as cellular redox state and oxygen concentration may affect photobleaching...

...the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in...

...https://doi.org/10.1038/nature22377 Harrington LB, Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B...

...occur. Similar systems include tetracycline or doxycycline mediated activation of Cre to either induce or...

...also choose to add smaller proteins, such as Thioredoxin A (TrxA) that improve disulfide bond formation...

...dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids...