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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33 results for: phr

Showing: 1 - 20 of 33 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ... activity. In 2019, the first generation of norepinephrine sensors was developed, named GRABNE, which ...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools ...limitations by developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki...indicators are more sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE... provide inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting...major advantages of this next generation of norepinephrine sensors is their speed. nLightG is activated...

  2. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...De-jargonizer.   Academic Phrasebank  The Academic Phrasebank is a resource for phrases commonly used in research...introduction, methods, results; or phrases are categorized by the goal of the phrase, such as being cautious, being... being critical, or defining terms. The phrases in Phrasebank are generic, so they need to be edited to...research papers or dissertations. Phrases are categorized either based on the section of a manuscript they...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...high spatiotemporal measurements of norepinephrine. These norepinephrine sensors include GRAB_NE1h (high ...from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors allow for sensitive and high...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...and triggering release of the stress hormone norepinephrine to promote vigilance. A recent study investigated...those neurons are chemogenetically activated, norepinephrine release causes a freezing response, a form ...associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals...

  5. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain (VP64...as directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the...performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65 - it had the lowest background activity in ...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... an AAV vector to express ChR2(H134R) in A2 norepinephrine expressing neurons. We tested four serotypes...dopamine beta-hydroxylase (DBH, magenta) to label norepinephrine (NE) expressing neurons and GFP (green) to ...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...cited below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through... labs of Jeffrey Chao, Robert Singer, and Anne Ephrussi allows researchers to observe when and where translation...

  12. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...

  17. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...thing’ could be a neurotransmitter, such as norepinephrine, or an electrolyte such as calcium. This varied...

  18. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal...
Showing: 1 - 20 of 33 results