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We narrowed to 46 results for: phr

Showing: 1 - 20 of 46 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ... activity. In 2019, the first generation of norepinephrine sensors was developed, named GRABNE, which ...which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools ...limitations by developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki...indicators are more sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE... provide inhibitory feedback in response to norepinephrine release, and clinically-used drugs targeting...major advantages of this next generation of norepinephrine sensors is their speed. nLightG is activated...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Stephen Royle 101223 pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford Brangwynne 101224 pHR-FUSN-mCh-Cry2olig FUS...122148 M23: pHR-SFFVp-FUSN::mCherry::SspB FUS mCherry SFFV ALS Clifford Brangwynne 122441 pHR-FUSN-miRFP670...Zhang 145269 pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS TBP SFFV Parkinson's Denes Hnisz 145270 pHR-TBP(53Q)-IDR-mCherry-Cry2... FUS SFFV ALS Clifford Brangwynne 101226 pHR-HNRNPA1C-mCh-Cry2WT HNRNPA1 SFFV ALS Clifford Brangwynne ...miRFP670 SFFV ALS Clifford Brangwynne 122668 M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB HNRNPA1 mCherry SFFV...SFFV ALS Clifford Brangwynne 122669 M31: pHR-SFFVp- TDP-43C::mCherry::SspB TARDBP mCherry SFFV ALS Clifford...

  3. Optogenetics Guide

    Type
    Guide
    ...CIB1 to control transcription effectors OPTOSTIM PHR domain2 of cryptochrome 2 (Cry2) from Arabidopsis...Increased photocurrent amplitude Examples: eNpHR, eNpHR2.0, eNpHR3.0 Red-shifted peak action spectra Example...pharaonis , used to silence neuronal activity 589 eNpHR 3.0 NpHR with addition of trafficking signal from Kir2.1... halobacteria. Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni ), causes hyperpolarization...yellow light, thus inhibiting function of the neuron. NpHR Variants. Variants have been engineered with enhancements...Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...

  4. Using a Light Microscope Protocol

    Type
    Protocol
    ...BioRender.com. Base Light Source Condenser and Diaphragm Stage Objective Lenses Focus Knobs (Fine and Coarse...by a) adjusting the aperture of the condenser diaphragm to limit the amount of light hitting the sample...the eyepiece comfortably. Then, close down the diaphragm and use the coarse focus adjustment knob (the ...in your field of view and then slowly open the diaphragm until the edges are just out of view. Pro-Tip ... the power of the light source by opening the diaphragm so that you can easily see your sample. Then use...

  5. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...De-jargonizer.   Academic Phrasebank  The Academic Phrasebank is a resource for phrases commonly used in research...introduction, methods, results; or phrases are categorized by the goal of the phrase, such as being cautious, being... being critical, or defining terms. The phrases in Phrasebank are generic, so they need to be edited to...research papers or dissertations. Phrases are categorized either based on the section of a manuscript they...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...high spatiotemporal measurements of norepinephrine. These norepinephrine sensors include GRAB_NE1h (high ...from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors allow for sensitive and high...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...and triggering release of the stress hormone norepinephrine to promote vigilance. A recent study investigated...those neurons are chemogenetically activated, norepinephrine release causes a freezing response, a form ...associated with psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain (VP64...as directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the...performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65 - it had the lowest background activity in ...

  10. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring...measurements of dopamine (113049, 113050) or norepinephrine (123308). The two dopamine sensors offer different...

  12. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... an AAV vector to express ChR2(H134R) in A2 norepinephrine expressing neurons. We tested four serotypes...dopamine beta-hydroxylase (DBH, magenta) to label norepinephrine (NE) expressing neurons and GFP (green) to ...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...Labeling in the Phrenic Motor System of Adult Rats: Intrapleural Injection versus Intradiaphragmatic Injection...

  14. Optogenetics AAV Preps

    Type
    Collection
    ...floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent 9 Karl Deisseroth 26966 AAV-Ef1a-DIO eNpHR 3.0-EYFP...CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Karl Deisseroth 26972 pAAV-hSyn-eNpHR 3.0-EYFP Syn eNpHR 3.0 EYFP...pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl Deisseroth 137152 pAAV-nEF-Con/Fon-NpHR3.3-EYFP... nEF NpHR 3.3 EYFP Cre dependent 8 Karl Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR 3.3 ...EYFP EF1a eNpHR 3.0 EYFP Cre dependent 1, 5, 9, rg* Karl Deisseroth 26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP ...EYFP nEF NpHR 3.3 EYFP Cre and Flp dependent 8 Karl Deisseroth 137153 pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP ... ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...floxed-eNpHR-EYFP-WPRE-pA Optogenetics Karl Deisseroth AV-9-26966P 26966-AAV9 pAAV-Ef1a-DIO eNpHR 3.0-EYFP...Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-1-26967M... Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-1-26973P...Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-5-26968P...Karl Deisseroth AV-5-26972P 26972-AAV5 pAAV-hSyn-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-5-26973P... Deisseroth AV-9-26971P 26971-AAV9 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth AV-9-26973P...Sternson AV-1-20949P 20949-AAV1 pAAV-double floxed-eNpHR-EYFP-WPRE-pA Karl Deisseroth AV-1-26967P 100050-...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Yulong Li 123309 pAAV-hSyn-GRAB_NE1h...genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Yulong Li 123310 pAAV-hSyn-GRAB_NEmut... GRAB-NEmut control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Yulong Li 20297...GCaMP6f GCaMP6s GECO Other Biosensors Glutamate Norepinephrine Dopamine Optogenetics Activators Inhibitors...Optogenetics Karl Deisseroth 26966 pAAV-Ef1a-DIO eNpHR 3.0-EYFP EF1a Inhibitor, Cre-dependent Optogenetics...

  17. Brain Initiative Collection

    Type
    Collection
    ...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123309...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123310...Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons Yulong...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...cited below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through... labs of Jeffrey Chao, Robert Singer, and Anne Ephrussi allows researchers to observe when and where translation...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic...
Showing: 1 - 20 of 46 results