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We narrowed to 23 results for: sds

Showing: 1 - 20 of 23 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...increasing the amount of SDS while decreasing the amount of alcohol. The increased SDS will increase the negative... subcellular compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few...transfers, you can adjust the amounts of alcohol and SDS in your transfer buffers. To slow down smaller proteins...the amount of alcohol and decrease the amount of SDS, with a decreased transfer time. Alcohol will slow...proteins to stick to the membrane by stripping away the SDS. To speed up larger proteins and prevent them from...the current.  Pro tip! You need both alcohol and SDS for your proteins to migrate…so don’t try eliminating... transfer time Increase voltage Increase SDS/decrease alcohol in transfer buffer Small proteins...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...purification. Figure 3: The Penn State Ladder run on SDS-Page gels. A: individually expressed proteins run...are purified via histidine tags, are optimized for SDS-PAGE gels and can be detected on Western blots via...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...electrophoresis (SDS-PAGE) is the first step of a western. To prepare the samples for SDS-PAGE, measure ...(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender AR (2019...

  4. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... or % Glycine or Glycine-HCl 25–200 mM SDS 0.1%–1% (w/v) Tween 20 (optional) 1% (v/v... % Purpose Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...in the cytoplasm or whole-cell lysate. A harsher, SDS-containing lysis buffer, like RIPA, should be considered...Bradford assays are not compatible with detergents like SDS, while BCA assays are not with denaturing agents ...proteins To denature your proteins, you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is... Table 1 lists the most common types of gels for SDS-PAGE running conditions and will cover most western...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ... is coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation of Mok and...antisense-RNA binds a sequence downstream of the mok SD resulting in a double stranded RNA structure that...

  7. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...separation of proteins by mass:charge ratio in an SDS-page gel. As the protein unfolds, new epitopes may...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...blot then it can be eluted by boiling the beads in SDS. (To learn more about Western blotting check out ...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... v1, vector 1; v2, vector 2; BD, binding domain; SDS, splice donor site; SAS, splice acceptor site; pA...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis of DNA topoisomers, knots, and catenanes...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...similary reduce proteolysis of the expressed proteins. hsdSB (rB- mB-): These strains have an inactivated native...IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) General protein expression...cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) pLysS (CamR) Expression...cannot have p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) pLysE (CamR) Expression...results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131 (DE3) General expression...may still be required for expression F- ompT lon hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA General protein...plasmids with repetative sequences.  F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR...

  18. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ....  Synthesis-dependent strand-annealing (SDSA) pathway SDSA is a conservative repair mechanism which ...repair (DSBR), synthesis-dependent strand-annealing (SDSA), and break-induced repair (BIR). The non-conservative...elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR is characterized... breaks that lack a second end to enable DSBR or SDSA.   Homology directed repair and genome engineering...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated DNA not degraded, cell cannot...). F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5...cloning and storage of pBR322 plasmids.  F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 ...additional wash step. F- glnV44 recA13 mcrB mrr hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5...recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F'[proAB lacIqZΔM15 Tn10(TetR Amy ...

  20. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search... search function that allows the user to find an MSDS for a lab chemical of choice. Now, I can’t guarantee...about a hazardous chemical and can’t find your lab’s MSDS book, this is a great resource to have in your pocket...Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional Resources on the Addgene Blog...
Showing: 1 - 20 of 23 results